Abstract
The self-association of nucleosides decreases within the series adenosine>guanosine>inosine>cytidine ≈uridine. The same trend is observed for the corresponding nucleotides, though less pronounced, as the charge effect governs series like adenosine ≫ AMP2−>ADP3−≳ATP4−. Protonation of adenosine considerably reduces its self-stacking tendency: this is different with ATP4−, where a maximum is reached for H2(ATP)2− caused by additional ionic interactions in the [H2(ATP)]2 4− dimer. Metal ion coordination may promote self-association, e.g., of ATP4− via (mainly) charge neutralization (Mg2+) and the formation of intermolecular bridges in dimeric stacks (Zn2+, Cd2+). These results allow definition of conditions with negligible self-association and thus the determination of the stability and structure of monomeric nucleotide complexes in aqueous solution, e.g., quantification of macrochelate formation in M(ATP)2− complexes. Some biological implications of the results are indicated.
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Sigel, H. Self-association of nucleotides. Biol Trace Elem Res 21, 49–59 (1989). https://doi.org/10.1007/BF02917236
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DOI: https://doi.org/10.1007/BF02917236