The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein)
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Was studied in vitro. The109Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd2+ from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.
Index EntriesMetallothionein (Cd-thionein), rat liver metallothionein, Cd-induced Cd-thionein, pH dependent dissociation lysosomal extract, rat liver lysosomal degradation of Cd-thionein, effect of pH on
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