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Biological Trace Element Research

, Volume 7, Issue 1, pp 49–54 | Cite as

The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein)

  • H. Meulenaar
  • C. J. A. van den Hamer
  • T. S. G. A. M. van den Ingh
  • T. M. W. Stolk
Original Articles

Abstract

Was studied in vitro. The109Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd2+ from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.

Index Entries

Metallothionein (Cd-thionein), rat liver metallothionein, Cd-induced Cd-thionein, pH dependent dissociation lysosomal extract, rat liver lysosomal degradation of Cd-thionein, effect of pH on 

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Copyright information

© The Humana Press Inc 1985

Authors and Affiliations

  • H. Meulenaar
    • 1
  • C. J. A. van den Hamer
    • 2
  • T. S. G. A. M. van den Ingh
    • 1
  • T. M. W. Stolk
    • 2
  1. 1.Department of Veterinary Pathology, Faculty of Veterinary MedicineState UniversityUtrechtThe Netherlands
  2. 2.Interuniversity Reactor InstituteDelftThe Netherlands

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