The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein)
Was studied in vitro. The109Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd2+ from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.
Index EntriesMetallothionein (Cd-thionein), rat liver metallothionein, Cd-induced Cd-thionein, pH dependent dissociation lysosomal extract, rat liver lysosomal degradation of Cd-thionein, effect of pH on
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- 1.J. H. R. Kägi and M. Nordberg, inMetallothionein, J. H. R. Kägi and M. Nordberg, eds., Exp. Supplementum, 34, Birkhäuser, Basel, 1979.Google Scholar
- 2.R. D. Anderson and U. Weser,Biochem. J. 175, 841 (1978).Google Scholar
- 3.F. O. Brady, M. Panemangalore, F. A. Day, A. M. Fiskin and G. Peterson, inMetallothionein, J. H. R. Kägi and M. Nordberg, eds., Exp. Supplementum 34, Birkhäuser, Basel, 1979, pp. 261–271.Google Scholar
- 10.U. Weser and H. Rupp, inMetallothionein, J. H. R. Kägi and M. Nordberg, eds., Exp. Supplementum 34, Birkhäuser, Basel, 1979, pp. 221–230.Google Scholar