Applied Biochemistry and Biotechnology

, Volume 43, Issue 3, pp 189–197 | Cite as

Improvement of culture conditions forl-proline production by a recombinant strain ofSerratia marcescens

  • Makoto Masuda
  • Satoru Takamatu
  • Noriyuki Nishimura
  • Saburo Komatsubara
  • Tetsuya Tosa


Serratia marcescens SP511 was previously reported to be anl-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L ofl-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions. We searched for more suitable culture conditions for more abundantl-proline production by SP511. To improve the supply of a nitrogen source to cells, ammonium was used instead of urea and fed to a culture under control of the pH of the medium. The concentrations of MgSO4 and K2HPO4 were increased, and in addition, sucrose was continuously added to the culture at a final concentration of 32%. Under these conditions, the cell amount was increased twofold over that under the previous conditions andl-proline production reached a maximum of more than 100 g/L after 4 d of incubation.

Index Entries

l-proline production nitrogen source ammonia Serratia marcescens 


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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Makoto Masuda
    • 1
  • Satoru Takamatu
    • 1
  • Noriyuki Nishimura
    • 1
  • Saburo Komatsubara
    • 1
  • Tetsuya Tosa
    • 1
  1. 1.Research Laboratory of Applied BiochemistryTanabe Seiyaku Co. Ltd.OsakaJapan

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