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Applied Biochemistry and Biotechnology

, Volume 41, Issue 3, pp 219–231 | Cite as

High-tech breakthrough DNA scanner for reading sequence and detecting gene mutation

A powerful 1 Ib, 20 μm resolution, 16-bit personal scanner (PS) that scans 17″×14″ X-ray film in 48 s, with laser, UV and white light sources
  • Jack A. Zeineh
  • Michael M. Zeineh
  • R. A. Zeineh
Article
  • 39 Downloads

Abstract

The 17″×14″ X-ray film, gels, and blots are widely used in DNA research. However, DNA laser scanners are costly and unaffordable for the majority of surveyed biotech scientists who need it. The high-tech breakthrough analytical personal scanner (PS) presented in this report is an inexpensive 1 lb hand-held scanner priced at 2–4% of the bulky and costly 30–95 lb conventional laser scanners. This PS scanner is affordable from an operation budget and biotechnologists, who originate most science breakthroughs, can acquire it to enhance their speed, accuracy, and productivity. Compared to conventional laser scanners that are currently available only through hard-to-get capital-equipment budgets, the new PS scanner offers improved spatial resolution of 20 μm, higher speed (scan up to 17″×14″ molecular X-ray film in 48 s), 1–32,768 gray levels (16-bits), student routines, versatility, and, most important affordability. Its programs image the film, read DNA sequences automatically, and detect gene mutation. In parallel to the wide laboratory use of PC computers instead of mainframes, this PS scanner might become an integral part of a PC-PS powerful and cost-effective system where the PS performs the digital imaging and the PC acts on the data.

Index Entries

DNA sequence electrophoresis fingerprinting PCR autoradiography 17″×14″ X-ray film archiving scanner 

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References

  1. 1.
    Davis, B. J. (1964),Ann. NY Acad. Sci. 121, 404–427.CrossRefGoogle Scholar
  2. 2.
    Ornstein, I. (1964),Ann. NY Acad. Sci. 121, 321–349.CrossRefGoogle Scholar
  3. 3.
    Zeineh, R. A., Nijm, W. P., and Al-Azzawi, F. (1975),Amer. Lab. 7(2), 51–68.Google Scholar
  4. 4.
    Zeineh, R. A. (1977), USA patent #4014610, issued March 29.Google Scholar
  5. 5.
    Crawford, C. R. and King, K. F. (1990),Med. Phys. (USA) Nov–Dec, 17(6), 967–982.Google Scholar
  6. 6.
    Zeineh, M. M. and Zeineh, R. A. (1990),Appl. Biochem. Biotechnol. 23, 81–90.CrossRefGoogle Scholar
  7. 7.
    Zeineh, R. A., Kyriakidis, S. G., Bhatti, A. R., and Webber, J. (1986),Appl. Biochem. Biotechnol. 12, 25–30.CrossRefGoogle Scholar
  8. 8.
    Wandtke, J. C. (1990),J. Thorac. Imaging (USA) Jan, 5(1), 1–9.Google Scholar
  9. 9.
    Picot, P. A., Cardinal, H. N., and Fenster, A. (1990),Med. Phys. (USA) Nov–Dec, 17(6), 983–988.Google Scholar
  10. 10.
    Kono, M., Yamasaki, K., and Ikeda, M. (1990),J. Thorac. Imaging (USA) Jan, 5(1), 61–66.CrossRefGoogle Scholar
  11. 11.
    Hansell, D. M. (1991),Curr. Opin. Radiol. (USA),Jun,3(3), 364–371.Google Scholar
  12. 12.
    MacMahon, H., Sanada, S., Doi, K., Giger, M., Hu, H. W., Yin, F. F., Montner, S. M., and Carlin, M. (1991),Radiographics (USA) Mar, 11(2), 259–268.Google Scholar
  13. 13.
    Wenzel, A. and Sewerin, I. (1991),Oral Surg. Med. Pathol. (USA) Apr. 71(4), 503–508.CrossRefGoogle Scholar

Copyright information

© Human Press Inc. 1993

Authors and Affiliations

  • Jack A. Zeineh
    • 1
  • Michael M. Zeineh
    • 2
  • R. A. Zeineh
    • 3
  1. 1.University of California, San DiegoLa Jolla
  2. 2.California Institute of TechnologyPasadena
  3. 3.Research Dept.AAB Advanced American BiotechnologyFullerton

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