Abstract
The release of alkaloids from root culturesDatura stramonium andCatharanthus roseus and thiophenes from root cultures ofTagetes patula was found to increase when the pH of the culture media (ranging from 4.8 to 7.0) was reduced to 3.5. The extent of the effect was different in each type of culture. Increases ranged from 4- to 20-fold, which in some cases accounted for 75% of the total secondary metabolite pool produced per flask. When the release of individual metabolites was measured, even larger increases, were observed (nearly 400-fold for ajmalicine). Increased release of alkaloids fromC. roseus roots were also observed in cultures growing in a 14-L fermentor, when the medium pH was reduced. Reduction of the pH of the media did not affect growth of the root cultures in subsequent subcultures. The importance of this treatment as a stategy to improve the recovery of secondary metabolites from producing cultures is discussed.
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References
Becker, H. and Sauerwein, M. (1990), inSecondary Products from Plant Tissue Culture, Charlwood, B.V. and Rhodes, M. J. C., eds., Oxford University Press, Oxford, pp. 43–57.
Parr, A. J. (1989),J. Biotechnol. 10, 1.
Guern, J., Renaudin, J. P., and Brown, S. C. (1987), inCell Cultures and Somatic Cell Genetics of Plants, vol. 4, Constabel, F. and Vasil, I. K., eds., Academic, San Diego, CA, pp. 43–76.
Parr, A. J., Robins, R. J., and Rhodes, M. J. C. (1987), inProcess Possibilities in Plant and Animal Cell Culture, Webb, C., and Mavituna, F., eds., Ellis Horwood, Chichester, pp. 229–237.
Buitelaar, R. M., Langenhoff, A. A. M., Heidstra, R., and Tramper, R. (1991),Enzyme Microbiol. Technol. 13, 487.
Parr, A. J., Robins, R. J., and Rhodes, M. J. C. (1984),Plant Cell Rep. 3, 262.
Rhodes, M. J. C., Hilton, M., Parr, A. J., Hamill, J. D., and Robins, R. J. (1986),Biotechnol. Lett. 8, 415.
Jardin, B., Tom, R., Chavarie, C., Rho, D., and Archambault, J. (1991),J. Biotechnol. 21, 43.
Gamborg, O. L. and Eveleigh, D. E. (1968),Can. J. Biochem. 46, 414.
Murashige, T. and Skoog, F. (1962),Physiol. Plant. 15, 473.
Monforte-González, M., Ayora-Talavera, T., Maldonado-Mendoza, I. E., and Loyola-Vargas, V. M. (1992),Phytochem. Anal. 3, 117.
Downum, K. R. and Towers, G. H. N. (1983),J. Nat. Prod. 46, 98.
Davis, K. R. and Hahlbrock, K. (1987),Plant Physiol. 85, 1286.
Payne, J., Hamill, J. D., Robins, R. J., and Rhodes, M. J. C. (1987),Planta Med. 53, 474.
Renaudin, J. P. and Guern, J. (1990), inSecondary Products from Plant Tissue Culture, Charlwood, B. V. and Rhodes, M. J. C., eds., Oxford University Press, Oxford, pp. 59–78.
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Sáenz-Carbonell, L.A., Maldonado-Mendoza, I.E., Moreno-Valenzula, O. et al. Effect of the medium pH on the release of secondary metabolites from roots ofDatura stramonium, Catharanthus roseus, andTagetes patula cultured in vitro. Appl Biochem Biotechnol 38, 257–267 (1993). https://doi.org/10.1007/BF02916405
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DOI: https://doi.org/10.1007/BF02916405