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Plant Molecular Biology Reporter

, Volume 24, Issue 2, pp 247–248 | Cite as

A method for the medium-term storage of plant tissue samples at room temperature and successive cycles of DNA extraction

  • Caroline Tassy
  • Catherine Feuillet
  • Pierre Barret
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Abstract

Based on the protocol originally described by Stein et al. (2001), we have developed a method that allows for medium-term conservation at room temperature of wheat (Triticum aestivum) tissue samples to use for DNA extraction. DNA quality was suitable for analysis by PCR and Southern hybridization, even after 2 months of storage at room temperature. This method allows successive DNA re-extractions from a previously extracted sample and maximization of the DNA yield that can be recovered from precious samples. This method has applications for conservation of leaf samples and management of DNA extraction. Our method can help improve data recovery in many plant molecular genetics research projects.

Key words

DNA extraction PCR sample conservation Southern blot wheat yield 

Copyright information

© Springer 2006

Authors and Affiliations

  • Caroline Tassy
    • 1
  • Catherine Feuillet
    • 2
  • Pierre Barret
    • 1
  1. 1.Plateforme de Transgénèse du BléUniversité Blaise PascalClermont-FerrandFrance
  2. 2.Structure, Fonction, et Évolution du Génome du Blé, UMR ASP 1095 INRAUniversité Blaise PascalClermont-FerrandFrance

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