In vitro propagation ofBoswellia serrata Roxb
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Anin vitro procedure for large scale multiplication ofBoswellia serrata Roxb. has been developed using cotyledonary node segments. In average 4 shoots per node were obtained on Murashige and Skoog’s (MS) medium containing 0.5 mg dm−3 6-benzylaminopurine (BAP) and 0.05 mg dm−3 napthaleneacetic acid (NAA) within 22 d. By repeated subculture technique 90–100 shoots per node could be obtained after 88 d of initial culture. Shoots could be rooted on MS medium containing 1/4 salts, 1% saccharose, and a combination of 0.5 mg dm−3 indole-3-butyric acid (IBA) and 0.25 mg dm−3 indole-3-acetic acid (IAA). Addition of antioxidants like polyvinylpyrrolidone (PVP-50 mg dm−3) and ascorbic acid (100 mg dm−3) in both multiplication and rooting media prevented browning of cultures. Approximately 80% of shoots rooted within 8–10 d. Rooted plantlets were kept for 15 d in culture bottles containing SoilriteTM irrigated with a nutrient solution containing 1/4 MS salts and finally transferred to pots containing soil: SoilriteTM (1∶1), mixture with 70% transplantation success.
Key wordsafforestation benzylaminopurine indole-3-acetic acid indole-3-butyric acid kinetin napthaleneacetic acid
Murashige and Skoog’s (1962) medium
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