Biologia Plantarum

, Volume 37, Issue 2, pp 297–300 | Cite as

Leaf bisection for the enzymatic isolation of mesophyll protoplasts fromSaintpaulia ionantha

  • M. A. Sulzinski
  • L. M. Cimakasky
Brief Communication


We describe a rapid and efficient procedure for isolating leaf mesophyll protoplasts fromSaintpaulia ionantha (African violet). Because of the unusual surface properties of the leaf, efficient digestion required bisection of the lamina into abaxial and adaxial halves to expose the mesophyll. Enzymatic digestion occurred during a 3 h incubation with 2% (m/v) driselase in 0.50 M mannitol, yielding 2–3 × 105 protoplasts g−1 (f.m.), with 70% viability. Protoplasts ranged in size from 50 to 100μm, and were derived from mostly non-photosynthetic mesophyll parenchyma.


African violet driselase mannitol viability 


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Copyright information

© Institute of Experimental Botany, ASCR 1995

Authors and Affiliations

  • M. A. Sulzinski
    • 1
  • L. M. Cimakasky
    • 1
  1. 1.Department of BiologyUniversity of ScrantonScrantonUSA

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