Indian Journal of Clinical Biochemistry

, Volume 21, Issue 1, pp 165–168 | Cite as

Impaired hypothalamic-pituitary-gonadal axis function in men with diabetes mellitus

  • M. Maneesh
  • H. Jayalakshmi
  • T A Singh
  • Amit Chakrabarti


In view of association of diabetes mellitus and male infertility, present study was designed to investigate the functional status of hypothalamic pituitary gonadal (HPG) axis in diabetic men. Thirty-five diabetic men (BMI 22.24±0.21) in the age group 20–40 (30.6±4.7) years were selected. Twenty-five healthy men (BMI 23.85±0.25), in the same age group (29.5±4.8) served as control. Blood samples were collected for hormonal and biochemical estimations. Diabetic men had significantly low serum testosterone with low LH and FSH, hypercholesterolemia, hypertriglyceridemia, hypoalbuminemia and increased oxidative stress. Low serum testosterone in diabetic men was accompanied by low LH and FSH; the inability of the pituitary gland to respond appropriately to a decline in testosterone implying central effect of high serum glucose on the interaction between the nervous and endocrine system. Nutritional deficiency, increased oxidative stress and increased aromatase activity due to excessive body fat might have also contributed to low serum testosterone.

Key words

Diabetes Mellitus Testosterone Hypothalamic-pituitary-gonadal axis Oxidative stress 


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  1. 1.
    Dinulovic, D. and Radonic, G. (1990). Diabetes mellitus and male infertility. Arch. Androl. 25, 277–293.PubMedCrossRefGoogle Scholar
  2. 2.
    Dunsmuir, W.D. and Holmes, S.A. (1996). The etiology and management of erectile, ejaculatory and fertility problems in men with diabetes mellitus. Diabet. Med. 13 (8), 700–708.PubMedCrossRefGoogle Scholar
  3. 3.
    Remzi Cevik, Ali Gur, Suat Acar, Kemal Nas and Ayoegül Jale Sarac. (2004). Hypothalamic-pituitary-gonadal axis hormones and cortisol in both menstrual phases of women with chronic fatigue syndrome and effect of depressive mood on these hormones. BMC Musculoskelet. Disord. 5, 47–51.PubMedCrossRefGoogle Scholar
  4. 4.
    Gil-Del Valle, L., Milian Lde, L., Toledo, A., Vilaro, N., Tapanes, R. and Otero, M.A. (2005). Altered redox status in patients with Diabetes Mellitus type I. Pharmacol. Res. 51(4), 375–380PubMedCrossRefGoogle Scholar
  5. 5.
    McCormick, D.B. and Greene, H.L. (1988). Tietz textbook of Clinical Chemistry. Eds. Burtis, C.A. and Ashwood, E.R. pp. 1025. W B Saunders Company, USA.Google Scholar
  6. 6.
    Sinhuber, R.O., Yu, T.C. and Yu, T.C. (1958). Characterization of the red pigment formed in the thiobarbituric acid determination of oxidative rancidity. Food Res. 23, 626–630.Google Scholar
  7. 7.
    Beutler, E., Duron, O. and Kelly, B.M. (1963). Improved method for determination of blood glutathione. J. Lab. Clin. Med. 61, 882–888.PubMedGoogle Scholar
  8. 8.
    Beers, R.F. and Sizer, I.W. (1952). A spectrophotometric method for measuring the break down of hydrogen peroxide by catalase. J. Biol. Chem. 195, 133–140.PubMedGoogle Scholar
  9. 9.
    Pinto, R.E. and Bartley, W. (1969). The effect of age and sex on glutathione reductase and glutathione peroxidase activities and on aerobic glutathione oxidation in rat liver homogenate. Biochem. J. 112, 109–115.PubMedGoogle Scholar
  10. 10.
    Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974). J. Biol. Chem. 249, 7130–7139.PubMedGoogle Scholar
  11. 11.
    Emanuele, M.A. and Emanuele, N. (2001). Alcohol and the male reproductive system. Alcohol Research & Health. 25 (4), 282–287.Google Scholar
  12. 12.
    Prichard, J., Despres, J.P., Gagnon, J., Tchernof, A., Nadeaci, A., Tremblag, A. and Bouchard, C. (1998). Journal of Clinical Endocrinology and Metabolisms 83 (9), 3277–3284.CrossRefGoogle Scholar
  13. 13.
    Das, S.K., Nayak, P. and Vasudevan, D.M. (2003). Biochemical markers of alcohol consumption, Ind. J. Clin. Biochem. 18 (2), 111–118.CrossRefGoogle Scholar
  14. 14.
    Shah Siddharth, N. (2000). Alpha lipic acid in diabetes, The Asian Journal of Diabetology 12, 17.Google Scholar
  15. 15.
    Maneesh, M., Jayalekshmi, H., Sanjiba Dutta, Amit Chakrabarti and Vasudevan, D.M. (2005). Effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity in rats. Indian J. Exp. Biol. (In Press).Google Scholar
  16. 16.
    Maneesh, M., Jayalekshmi, H., Sanjiba Dutta, Amit Chakrabarti and Vasudevan, D.M. (2005). Experimental therapeutical intervention with ascorbic acid in ethanol induced testicular injuries in rats. Indian J. Exp. Biol. 43, 172–176.PubMedGoogle Scholar
  17. 17.
    Mari, M., Wu, D., Nieto, N. and Cederbaum, A.I. (2001). CYP2E1-Dependent Toxicity and Up-Regulation of Antioxidant Genes. J. Biomed. Sci. 8 (1), 52–56.PubMedCrossRefGoogle Scholar
  18. 18.
    Irvine, D.S. (1996). Glutathione as a treatment for male infertility. Reprod. 1, 6–10.CrossRefGoogle Scholar
  19. 19.
    Asok Kumar, P. and Rajagopal, G. (2003). Lipid peroxidation in erythrocytes of patients with type 2 diabetes mellitus. Ind. J. Clin. Biochem. 18 (1), 71–74.CrossRefGoogle Scholar
  20. 20.
    Lenzi, A., Cualosso, F., Gandini, L., Lombardo, F. and Dondero, F. (1993). Placebo controlled; double-blind cross over trial glutathione therapy, in male infertility. Hum. Reprod. 9, 2044Google Scholar
  21. 21.
    Calvin, H.I., Cooper, G.W. and Wallace, E. (1981). Evidence that selenium in rat sperm is associated with a cystine rich structural protein of the mitochondrial capsule. Gamete Res. 4, 139–145.CrossRefGoogle Scholar
  22. 22.
    Chandra, R., Aneja, R., Rewal, C., Konduri, R., Dass, K. and Agarwal, S. (2000). An opium alkaloid-Papaverine ameliorates ethanol induced hepatotoxicity: diminution of oxidative stress. Ind. J. Clin. Biochem. 15 (2), 155–160.CrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2006

Authors and Affiliations

  • M. Maneesh
    • 1
  • H. Jayalakshmi
    • 2
  • T A Singh
    • 2
  • Amit Chakrabarti
    • 3
  1. 1.Department of BiochemistryMelaka Manipal Medical CollegeManipal
  2. 2.Department of BiochemistrySikkim Manipal Institute of Medical SciencesGangtok
  3. 3.Department of PharmacologySikkim Manipal Institute of Medical SciencesGangtok

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