Virchows Archiv B

, Volume 17, Issue 1, pp 229–238 | Cite as

Ultrastructural study of the relationship between immune cytotoxic lymphoid cells and target cellsin vitro

  • H. Firket
  • G. Degiovanni


Ultrastructural details of the relationshipin vitro between sensitized T lymphoid cells and YAC target cells are described. Within a few hours very close connections develop between these two cells. Narrow junctions with parallel plasma membranes and local evaginations of the lymphoid cell into recesses of the target cell are specific aspects. Very fine threadlike material is found between the two plasma membranes at the narrowest zones. The lymphoid cells concerned have an intermediate structure between ordinary small lymphocytes and blast cells. They do not seem to be modified in the process while YAC target cells are severely damaged. Polysomes are dispersed; mitochondria and rough endoplasmic reticulum are affected locally or generally. Later, nucleoli and chromatin become dense and pycnosis occurs.

The significance of these results is discussed.

Key words

Ultrastructure Lymphoid Cells Cellular Immunity Culture 


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  1. Able, M. E., Lee, J. C., Rosenau, W.: Lymphocyte-target cell interactionin vitro. Amer. J. Path.60, 421–434 (1970).PubMedGoogle Scholar
  2. Betz, E. H., Firket, H., Simar, L. J.: Mécanismes cellulaires de l’immunité de transplantation. Application à des tumeurs expérimentalesin vivo etin vitro. C. R. Ass. Anat. (sous presse) (1974)Google Scholar
  3. Biberfeld, P., Biberfeld, G., Perlmann, P., Holm, G.: Cytological observations on the cytotoxic interaction between lymphocytes and antibody-coated monolayer cells. Cell. Immunol.7, 60–72 (1973)PubMedCrossRefGoogle Scholar
  4. Cerottini, J. C., Brunner, T.K.:In vitro assay of target cells by sensitized lymphocytes. In: Bloom, B. R., and Glade, P. R. (eds.),In vitro methods in cell mediated immunity, p. 369. New York and London: Academic Press 1971Google Scholar
  5. Degiovanni, G.: Le mercaptoéthanol facilite,in vitro, l’apparition de lymphocytes cytotoxiques contre des cellules allogéniques. C. R. Soc. Biol. (Paris)167, 391–394 (1973)Google Scholar
  6. Firket, H., Degiovanni, G.: Ultrastructural aspects of specific immune cytotoxicity against tumour cellsin vitro. Joint Session on Electron Microscopy, Liège, September 1973Google Scholar
  7. Haÿry, P., Andersson, L. C., Nordling, S., Virolainen, M.: Allograft responsein vitro. Transplant. Rev.12, 91–140 (1972)PubMedGoogle Scholar
  8. Henney, C. S.: On the mechanism, of T cell mediated cytolysis. Transplant. Rev.17, 37–70 (1973)PubMedGoogle Scholar
  9. Koren, H. S., Ax, W., Freund-Molbert, E.: Morphological observations on the contact induced lysis of target cells. Europ. J. Immunol.3, 32–37 (1973)CrossRefGoogle Scholar
  10. Weiss, L.: Interaction of sensitized lymphoid cells and homologous target cells in tissue culture and grafts: an electron microscope and immunofluorescence study. J. Immunol.101, 1346–1362 (1968)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • H. Firket
    • 1
  • G. Degiovanni
    • 2
  1. 1.Laboratoire d’Anatomie PathologiqueUniversité de LiègeLiègeBelgium
  2. 2.Département de Chirurgie ExpérimentaleLiègeBelgium

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