Direct analysis of trivernolin by high-performance liquid chromatography

  • R. D. Plattner
  • K. Wade
  • R. Kleiman


A rapid liquid chromatographic method for the quantitation of trivernolin in epoxy seed oils has been developed. A weighed sample of the oil is dissolved in chloroform (∼100–300 mg/ml), and a 1–20µl portion of the oil is chromatographed on a 2 ft×1/4 in. OD C18 µ-Bondapak column with acetonitrile-acetone (2:1, v/v). The area of the trivernolin peak from the differential refractometer recorder tracing is converted toµg trivernolin by use of a calibration curve of peak area vs. trivernolin weight.


Stearin Differential Refractometer Epoxy Acid Vernolic Acid Octadecyl Silane 
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  1. 1.
    Krewson, C.F., JAOCS 45:250 (1968).CrossRefGoogle Scholar
  2. 2.
    Kleiman, R., C.R. Smith, and S.G. Yates, Ibid. 42:169 (1965).CrossRefGoogle Scholar
  3. 3.
    Fioriti, J.A., M.J. Kanuk, and R.J. Sims, J. Chromatogr. Sci. 7:448 (1969).Google Scholar
  4. 4.
    R.D. Plattner, G.F. Spencer, and R. Kleiman, JAOCS (In press).Google Scholar

Copyright information

© The American Oil Chemists’ Society 1978

Authors and Affiliations

  • R. D. Plattner
    • 1
  • K. Wade
    • 1
  • R. Kleiman
    • 1
  1. 1.Northern Regional Research Center, Agricultural Research ServiceU.S. Department of AgriculturePeoria

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