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Ricerca in clinica e in laboratorio

, Volume 5, Issue 2, pp 97–104 | Cite as

The assay of exocrinous peptidases in clinical chemistry

  • Marc Roth
Editorials
  • 16 Downloads

Summary

The various peptidases secreted by such exocrine tissues as gastric mucosa, pancreas and prostate are usually determined by catalytic methods. Another approach utilizes immunoassay. Endopeptidases were formerly assayed with protein substrates such as hemoglobin and albumin. These techniques are increasingly replaced by more specific ones using artificial peptide derivatives as substrates, some of which allow an increase in absorbance or fluorescence to be continuously recorded. The presently available methods of assaying pepsin, pancreatic trypsin, trypsinogen and carboxypeptidase A, enterokinase and several peptidases of human sperm are reviewed.

Key-words

Carboxypeptidases Chymotrypsin Duodenal juice Enterokinase Gastric juice Pancreas Pepsin Peptidases Prostate Sperm Trypsin 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Marc Roth
    • 1
  1. 1.Laboratoire CentralHôpital CantonalGenève 4Switzerland

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