Chinese Science Bulletin

, Volume 45, Issue 4, pp 338–343 | Cite as

Cloning, sequencing and structural analysis of a pea cDNA encoding EF-1α



A cDNA library with the capacity of 2.0 × 106 was constructed using mRNA extracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5′ non-coding region and 223 nucleotides of 3′ non-coding region was obtained by random screening. The DNA fragment contains an open reading frame of 1 344 nucleotides and encodes 447 amino acids. Sequence analysis of DNA and deduced polypeptide demonstrated that it encoded for a pea elongation factor 1-alpha (EF-1α). The functional domains of EF-1α is well conserved and EF-1α can be a good candidate for studying molecular evolution.


pea cDNA library EF-1α sequence analysis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Riis, B., Rattan, S. L. S., Clark, B. F. C. et al., Eukaryotic protein elongation factors, TIBS, 1990, 15: 420.PubMedGoogle Scholar
  2. 2.
    Shiina, N., Gotoh, Y., Kubomura, N. et al., Microtubule severing by elongation factor-1α, Science, 1994, 266: 282.PubMedCrossRefGoogle Scholar
  3. 3.
    Clore, A. M., Dannenhoffer, J. M., Larkins, B. A., EF-1α is associated with a cytoskeletal network surrounding protein bodies in maize endosperm cells, The Pant Cell, 1996, 8: 2003.CrossRefGoogle Scholar
  4. 4.
    Habben, J. E., Moro, G. L., Hunter, B. G. et al., Elongation factor-1α concentration is highly correlated with the lysine content of maize endosperm, Proc. Natl. Acad. Sci. USA, 1995, 92: 8640.PubMedCrossRefGoogle Scholar
  5. 5.
    Axelos, M., Bardet, C., Liboz, T. et al., The gene family encoding theArabidopsis thaliana translation elongation factor EF-1α: Molecular cloning, characterization and expression, Mol. Gen. Genet., 1989, 219: 106.PubMedCrossRefGoogle Scholar
  6. 6.
    Bonafonte, M. T., Preist, J. W., Garmon, D. et al., Isolation of the gene coding for elongation factor-1α inCryptosporidium parvum, Biochim. et. Biophys. Acta, 1997, 1351: 256.Google Scholar
  7. 7.
    Pokalsky, A. R., Hiatt, W. R., Ridge, N. et al., Structure and expression of elongation factor-1α in tomato, Nucl. Acids Res., 1989, 17: 4661.PubMedCrossRefGoogle Scholar
  8. 8.
    Qing, Y. L., Baldauf, S. L., Reith, M. E., Elongation factor-1α genes of the red algaPorphyra purpurea include a novel, developmentally specialized variant, Plant. Molecular Biology, 1996, 31: 77.CrossRefGoogle Scholar
  9. 9.
    Durso, N. A., Cyr, R. J., A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1α, The Plant Cell, 1994, 6: 893.PubMedCrossRefGoogle Scholar
  10. 10.
    Kurasawa, Y., Hanyu, K., Watanabe, Y. et al., F-actin bundling activity ofTetrahymena elongation factor 1α is regulated by Ca2+/calmodulin, J. Biochem., 1996, 119: 791.PubMedGoogle Scholar
  11. 11.
    Thompson, J. D., Higgins, D. G., Gibson, T. J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrixchoice, Nucleic Acids Research, 1994, 22: 4673.PubMedCrossRefGoogle Scholar
  12. 12.
    Baum, B. R., PHYLIP: Phylogeny Inference Package, Version 3.2. (Software review), Quarterly Review of Biology, 1989, 64: 539.CrossRefGoogle Scholar
  13. 13.
    Zhu, J. K., Damsz, B., Andrzej, K. et al., A higher plant extracellular vitronectin-like adhesion protein is related to the translational elongation factor-1α, The Plant Cell, 1994, 6: 393.PubMedCrossRefGoogle Scholar
  14. 14.
    Zhu, J. K., Damsz, B., Andrzej, K. et al., A higher plant extracellular vitronectin-like adhesion protein is related to the translational elongatio P. J., The control of apical bud growth and senescence by auxin and gibberellin in genetic lines of peas, Plant Physiol., 1997, 113: 631.PubMedGoogle Scholar
  15. 15.
    Zhu, Y. X., Zhang, Y. F., Luo, J. et al., PPF-1, a post-floral-specific gene expressed in short-day grown G2 pea may be important for the maintenance of vigorous growth, Gene, 1998, 208: 1.PubMedCrossRefGoogle Scholar

Copyright information

© Science in China Press 2000

Authors and Affiliations

  1. 1.National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life SciencePeking UniversityBeijingChina

Personalised recommendations