Chinese Science Bulletin

, Volume 45, Issue 4, pp 338–343 | Cite as

Cloning, sequencing and structural analysis of a pea cDNA encoding EF-1α

  • Xiangyu Cong
  • Shiyun Ling
  • Yuxian Zhu


A cDNA library with the capacity of 2.0 × 106 was constructed using mRNA extracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5′ non-coding region and 223 nucleotides of 3′ non-coding region was obtained by random screening. The DNA fragment contains an open reading frame of 1 344 nucleotides and encodes 447 amino acids. Sequence analysis of DNA and deduced polypeptide demonstrated that it encoded for a pea elongation factor 1-alpha (EF-1α). The functional domains of EF-1α is well conserved and EF-1α can be a good candidate for studying molecular evolution.


pea cDNA library EF-1α sequence analysis 


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Copyright information

© Science in China Press 2000

Authors and Affiliations

  1. 1.National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life SciencePeking UniversityBeijingChina

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