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Percoll density gradient centrifugation

An improved method for the enrichment of lymphocyte subsets mediating different activities
  • Donato Fumarola
  • Salvatore Antonaci
  • Emilio Jirillo
  • Irene Munno
  • Giacomo Lucivero
  • Lorenzo Bonomo
Original Contributions
  • 912 Downloads

Summary

Lymphocyte fractions obtained by Percoll density gradient centrifugation were assayed for their cytotoxic activities both in NK and ADCC cytotoxic systems. Furthermore, the release of a mediator, platelet slowing factor from the same subsets, was evaluated. Different mitogens (phytohemagglutinin, concanavalin A and lipid A) have been used for this purpose. The maximum of cytotoxic activities was recovered in E+ cell-enriched fractions. On the other hand, lipid A was able to stimulate the PSF production from enriched B cells (fraction I); phytohemagglutinin induced the release of PSF from E+ fractions (II and III) and concanavalin A from fraction II only. Our results suggest that Percoll density gradient is a very reproducible method in obtaining lymphocyte subsets enriched in cells mediating either cytotoxic activities or lymphokine release.

Key-words

Antibody-dependent cytotoxicity E-rosettes EA-rosettes Lymphokines Natural killer cytotoxicity Percoll Platelet slowing factor 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Donato Fumarola
    • 1
  • Salvatore Antonaci
    • 2
  • Emilio Jirillo
    • 1
  • Irene Munno
    • 1
  • Giacomo Lucivero
    • 2
  • Lorenzo Bonomo
    • 2
  1. 1.Istituto di Microbiologia MedicaUniversità degli Studi di Bari PoliclinicoBariItalia
  2. 2.Istituto di Clinica Medica Generale e Terapia Medica IUniversità degli Studi di BariItalia

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