Biologia Plantarum

, 23:16 | Cite as

Effects of salt stress on nitrogenase activity and growth of four legumes

  • A. M. Abdel Wahab
  • H. H. Zahran
Original Papers


The effects of salt stress on nitrogenase (N2-ase) activity, growth and nitrogen content ofVicia faba (L.),Medicago sativa (L.) Merrill,Glycine max andVigna sinensis (L.) were investigated. Four levels of salinity were applied and salt treatments were imposed on inoculated and N-fertilized plants.M. sativa tolerated mild levels of salinity but higher salt concentrations depressed N2-ase activity of this species. The other three legumes were considerably affected by salt treatments, and N2-ase activity was significantly reduced by salinity.

Vicia faba, carrying elongate nodules, could restore a partial N2-ase activity upon recovery from salt stress whereasG. max andV. sinesis, both with spherical nodules, could not regain significant activity when salinity was removed.

Salt stress retarded growth of both inoculated and N-fertilized plants. The nitrogen content of both treatments was also affected by salinity and the effect was more severe for inoculated than N-fertilized plants.


Salt Stress Nitrogen Fixation Salt Treatment C2H2 Reduction Faba Plant 
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  1. Abdel Wahab, A. M., Zahran, H. H.: The effect of water stress on N2(C2H2) fixation and growth ofMedicago sativa.-Aota agron. (In press), 1980.Google Scholar
  2. Allen, O. M.: Experiments in Soil Bacteriology.-Burgess Publishing Co., Minneapolis 1959.Google Scholar
  3. Balasubramanian, V., Sinha, S. K.: Effect of salt stress on growth nodulation, and nitrogen fixation in cowpea and mung beans.-Physiol. Plant.36: 197–200, 1976.CrossRefGoogle Scholar
  4. Bernstein, L., Ogata, G.: Effects of salinity on nodulation, nitrogen fixation, and growth of soybeans and alfalfa.-Agron. J.58: 201–203, 1966.Google Scholar
  5. Engin, M., Sprent, J. I.: Effects of water stress on growth and nitrogen-fixing activity ofTrifolium repens.-New Phytol.72: 117–126, 1973.CrossRefGoogle Scholar
  6. Greenway, H.: Effects of slowly permeating osmotica on metabolism of vacuolated and nonvacuolated tissue.-Plant Physiol.46: 254–259, 1970.PubMedCrossRefGoogle Scholar
  7. Hardy, R. W. F., Burns, R. C, Herbert, R. R., Holsten, R. D., Jackson, E. K.: Biological nitrogen fixation a key to world protein.-Plant Soil, Spec. Vol.: 561–590, 1971.Google Scholar
  8. Hardy, R. W. F., Holsten, R. D., Jackson, E. K., Burns, R. C.: The acetylene-ethylene assay for nitrogen fixation: laboratory and field evaluation.-Plant Physiol.43: 1185–1207, 1968.PubMedGoogle Scholar
  9. Poljakoff-Mayber, A., Gale, J.: Ecological Studies 15. Plants in Saline Environments. Springer Verlag, Berlin-Heidelberg-New York 1975.Google Scholar
  10. Sprent, J. I.: The effect of water stress on nitrogen-fixing root nodules. II. Effects on the fine structure of detached soybean nodules. -New Phytol.71: 443–450, 1972a.CrossRefGoogle Scholar
  11. Sprent, J. I.: The effect of water stress on nitrogen-fixing root nodules. III. Effects on osmotically applied stress. -New Phytol.71: 451–460, 1972b.CrossRefGoogle Scholar
  12. Sprent, J. I.: Water deficits and nitrogen-fixing root nodules. - In:Kozlowski, T. T. (ed.): Water Deficits and Plant Growth. Vol. IV. Pp. 291–315. Acad. Press, New York-London 1976.Google Scholar
  13. Stewart, W. D. P., Fitzgerald, G. P., Burris, R. H.:In situ studies on N2-fixation using the acetylene reduction technique. -Proc. nat. Acad. Sci. USA58: 2071–2078, 1976.CrossRefGoogle Scholar
  14. Subbarao, N. S., Lakshmi-Kumari, M., Singh, C. S., Magu, S. P.: Nodulation of lucerne (Medicago sativa L.) under the influence of sodium chloride.-Indian. J. agr. Sci.42: 385–386, 1972.Google Scholar
  15. Wilson, J. R.: Response to salinity inGlycine. Some effects of a range of short-term salt stress on the growth nodulation, and nitrogen fixation ofGlycine wigktii (formerlyjavanica). -Aust. J. agr. Res.21: 571–682, 1970.CrossRefGoogle Scholar
  16. Wilson, J. R., Norris, D. O.: Some effects of salinity onGlycine javanica and itsRhizobium symbiosis.-In: Proceedings of the XI International Grassland Congress. Pp. 455–468. Paradise, Queensland, Australia, 1970.Google Scholar
  17. Zahran, H. H.: Studies on the effect of water stress and salinity on nitrogen fixation by some legumes.-M. So. Thesis, Assiut Univ. 1978.Google Scholar

Copyright information

© Academia 1981

Authors and Affiliations

  • A. M. Abdel Wahab
    • 1
  • H. H. Zahran
    • 1
  1. 1.Department of BotanyAssiut UniversityAssiutEgypt

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