Summary
Amniotic fluid testosterone levels were determined by the radioimmunoassay technique in 101 samples obtained by amniocentesis between 16 and 18 weeks of gestation from women with increased risk of chromosomal abnormalities. Fetal sex was determined by cell culture and chromosomal analysis of fetal amniotic cells and was confirmed at birth. In pregnancies with male fetuses (50 samples), the mean amniotic fluid testosterone level of 216 pg/ml (±75) was significantly higher (p<0.001) than the mean value of 93 pg/ml (±28) found in pregnancies with female fetuses (51 samples). The range for pregnancies with male fetuses was 130 to 500 pg/ml and for those with female fetuses 10 to 140 pg/ml, showing an area of overlap from 130 to 140 pg/ml.
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References
Abramovich D. R., Rowe P.: Fetal plasma testosterone levels at mid-pregnancy and at term: relationship to fetal sex — J. Endocr.56, 621, 1973.
Acevedo H. F., Axelrod L. R., Ishikawa E., Takaki F.: Studies in fetal metabolism. II. Metabolism of progesterone-4-14C and pregnenolone 7α-3H in human fetal testis — J. clin. Endocr.23, 885, 1963.
Baille A. H., Ferguson M. M., Hart M. C. K.: Histochemical evidence of steroid metabolism in the human genital ridge — J. clin. Endocr.26, 738, 1966.
Belisle S., Fencl M., Tulchinsky D.: Amniotic fluid testosterone and follicle-stimulating hormone in the determination of fetal sex — Amer. J. Obstet. Gynec.128, 514, 1977.
Blizzard R. M., Alberts M.: Hypopituitarism, hypoadrenalism and hypogonadism in the newborn infants — J. Pediat.48, 782, 1956.
Bloch E.: Metabolism of 4-14C-progesterone by human fetal testis and ovaries — Endocrinology74, 833, 1964.
Brewer D.: Congenital absence of the pituitary gland and its consequences — J. Path. Bact.73, 59, 1957.
Clements J. A., Reyes F. I., Winter J. S. D., Faiman C.: Studies on human sexual development. III. Fetal pituitary, serum and amniotic fluid concentration of LH, HCG and FSH — J. clin. Endocr.42, 9, 1976.
Dörner G., Stahl F., Baumgarter G.: Signifikante Unterschiede in Testosteron und 11-OHCS-Gehalt des Fruchtwassers zwischen männlichen und weiblichen Föten — Endokrinologie60, 285, 1972.
Giles H. R., Lox C. D., Wayne Heine M., Christian C. D.: Intrauterine fetal sex determination by radioimmunoassay of amniotic fluid testosterone — Gynec. Invest.5, 317, 1974.
Gillman J.: The development of the gonads in man with a consideration of the role of the fetal endocrines and the histogenesis of ovarian tumors — Contr. Embryol. Carneg. Instn32, 81, 1948.
Goldman H. S., Yakovac W. C., Bongiovanni A. M.: Development of activity of 3β-hydroxy-steroid dehydrogenase in human fetal tissue and two anencephalic newborns — J. clin. Endocr.26, 14, 1996.
Jost A.: Recherches sur la différenciation sexuelle de l’embryon de lapin. II. Action des androgènes de synthèse sur l’histogénèse génitale — Arch. Anat. micr. Morph. exp.36, 242, 1947.
Jost A.: Recherches sur la différenciation sexuelle de l’embryon de lapin. III. Rôle des gonades foetales dans la différenciation sexuelle somatique — Arch. Anat. micr. Morph. exp.36, 271, 1947.
Judd H. L., Robinson J. D., Young P. E., Jones O. W.: Amniotic fluid testosterone levels in midpregnancy — Obstet. and Gynec.48, 690, 1976.
Judd H. L., Yen S. S. C.: Serum androstenedione and testosterone levels during the menstrual cycle — J. clin. Endocr.36, 475, 1973.
Klinga K., Bek E., Runnebaum B.: Maternal peripheral testosterone levels during the first half of pregnancy — Amer. J. Obstet. Gynec.131, 60, 1978.
Mancini R. E., Vilar O., Lavieri J. C., Andrada J. A., Heinrich J. J.: Development of Leydig cells in the normal human testis — Amer. J. Anat.112, 203, 1963.
Nagamani M., McDonough P., Ellegood J., Mahesh V. B.: Maternal and amniotic fluid steroids throughout human pregnancy — Amer. J. Obstet. Gynec.134, 674, 1979.
Pelliniemi L. J., Niemi M.: Fine structure of human fetal testis — Z. Zellforsch.99, 507, 1969.
Pirani B. B. K., Pairaudeau N., Doran T. A., Wong P. Y., Gardner H. A.: Amniotic fluid testosterone in the prenatal determination of fetal sex — Amer. J. Obstet. Gynec.129, 518, 1977.
Reid J. D.: Congenital absence of the pituitary gland — J. Pediat.56, 658, 1960.
Reyes F. I., Winter J. S. D., Faiman C.: Studies on human sexual development. I. Fetal gonadal and adrenal sex steroids — J. clin. Endocr.37, 74, 1973.
Rice B. F., Johnanson C. A., Stenberg W. H.: Formation of steroid hormones from acetate-1-14C by a human fetal testis preparation grown in organ culture — Steroids7, 79, 1966.
Robinson J. D., Judd H. L., Young P. E., Jones O. W., Yen S. S. C.: Amniotic fluid androgens and estrogens in mid-gestation — J. clin. Endocr.45, 755, 1977.
Serra G. V., Pérez-Palacios T., Jaffe R. B.:De novo testosterone biosynthesis in the human fetal testis — J. clin. Endocr.30, 128, 1970.
Wu C., Mennuti M., Mikhail G.: Free and protein-bound steroids in amniotic fluid of pregnancy — Amer. J. Obstet. Gynec.133, 666, 1979.
Zaaijer J. J., Price D.: Early secretion of androgenic hormones by human fetal gonads and adrenal glands in organ culture and possible complications for sex differentiation. In:Hamburgh M., Barrington E. J. W. (Eds): Hormones in development. Appleton-Century-Crofts, New York, 1971; p. 537.
Zondek L. H., Zondek T.: Observations on the testis in anencephaly with special reference to the Leydig cells — Biol. Neonat. (Basel)8, 329, 1965.
Zondek T., Mansfield M. D., Zondek L. H.: Amniotic fluid testosterone and fetal sex determination in the first half of pregnancy — Brit. J. Obstet. Gynec.84, 714, 1977.
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Ansaldi, E., Voglino, G., Coppo, F. et al. Amniotic fluid testosterone and fetal sex determination. La Ricerca Clin. Lab. 11, 349–354 (1981). https://doi.org/10.1007/BF02909034
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DOI: https://doi.org/10.1007/BF02909034