Biologia Plantarum

, 37:621 | Cite as

Involvement of phenolic acids in disease resistance of potato tubers from CEPA-treated plants

  • M. Cvikrová
  • J. Eder
  • L. S. Sukhova
  • N. P. Korableva
Original Papers


Treatment of vegetative parts of potato plants two weeks before the harvest with 0.2% 2-chloroethylphosphonic acid (CEPA) delayed the sprouting of tubers and increased the resistance of tubers to infections caused byPhytophthora infestans, Erwinia carotovora andFusarium spp. during the storage period. Levels of free, soluble ester- and glycoside-bound phenolic acids and cell wall-bound phenolics were determined in cortical parenchyma of tubers (periderm). The enhancement of phenolic acids in tubers from treated plants was caused primarily by the increase in the contents of free vanillic, caffeic andp-hydroxybenzoic acids and cell wall-bound ferulic, vanillic andp-coumaric acids.

Key words

2-chloroethylphosphonic acid resistance to diseases Solanum tuberosum 


  1. Ampomah, Y.A., Friend, J.: Insoluble phenolic compounds and resistance of potato tuber discs toPhytophthora andPhoma.—Phytochemistry27: 2533–2541, 1988.CrossRefGoogle Scholar
  2. Clarke, D.D.: The accumulation of cinnamic acid amides in the cell walls of potato tissue as an early response to fungal attack.—In: Wood, R.K.S. (ed.): Active defense mechanisms in plants. Pp. 321–322. Plenum Press, New York 1982.Google Scholar
  3. Clerivet, A., Macheix, J.J.: Interaction hôte-parasiteSolanum gilo (Raddli)Stemphylium floridanum (Hannon et Weber). Relation entre teneur en acide chlorogénique foliaire et résistance au pathogène.—Phytopathology139: 322–328, 1993.CrossRefGoogle Scholar
  4. Coleman, W.K., Murphy, A.: Effect of dormancy releasing chemicals on subsequent tuber response toFusarium sambucinum.—Amer. Potato J.67: 133–136, 1990.Google Scholar
  5. Cvikrová, M., Meravý, L., Macháčková, I., Eder, J.: Phenylalanine ammonia-lyase, phenolic acids and ethylene in alfalfa (Medicago sativa L.) cell cultures in relation to their embryogenic ability.— Plant Cell Rep.10: 251–255, 1991.CrossRefGoogle Scholar
  6. Cvikrová, M., Hrubcová, M., Vágner, M., Macháčková, I., Eder, J.: Phenolic acids and peroxidase activity in alfalfa (Medicago sativa) embryogenic culture after ethephon treatment.—Physiol. Plant.91: 226–233, 1994.CrossRefGoogle Scholar
  7. Ecker, J.R., Davis, R.W.: Plant defense genes are regulated by ethylene.—Proc. nat. Acad. Sci. USA84: 5202–5206, 1987.PubMedCrossRefGoogle Scholar
  8. Fuchs, A., De Vries, F.W.: Metabolism of radioactively labelled quinic acid and shikimic acid in healthy andFusarium-infected tomato plants.—Neth. J. Plant Pathol.75: 186–192, 1969.CrossRefGoogle Scholar
  9. Hahlbrock, K., Scheel, D.: Physiology and molecular biology of phenylpropanoid metabolism.— Annu. Rev. Plant Physiol. Plant mol. Biol.40: 347–369, 1989.CrossRefGoogle Scholar
  10. Hammerschmidt, R.: Rapid deposition of lignin in potato tuber tissue as a response to fungi non-pathogenic on potato.—Physiol. Plant Pathol.24: 33–42, 1984.Google Scholar
  11. Hammerschmidt, R.: Determination of natural and wound-induced potato tuber suberin phenolics by thioglycolic acid derivatization and cupric oxide oxidation.—Potato Res.28: 123–127, 1985.CrossRefGoogle Scholar
  12. Henderson, S.J., Friend, J.: Increase in PAL and lignin-like compounds as race-specific resistance response of potato tubers toPhytophthora infestans.—Phytopathol. Z.56: 323–334, 1979.CrossRefGoogle Scholar
  13. Hyodo, H., Kuroda, H., Yang, S.F.: Induction of phenylalanine ammonia-lyase and increase in phenolics in lettuce leaves in relation to the development of russet spotting caused by ethylene.— Plant Physiol.62: 31–35, 1978.PubMedGoogle Scholar
  14. Korableva, N.P., Sukhova, L.S., Dogonadze, M.Z., Macháčková, I.: Hormonal regulation of potato tuber dormancy and resistance to pathogens.—In: Krekule J., Seidlová, F. (ed.): Signals in Plant Development. Pp. 65–71. SPB Academic Publishing, The Hague 1989.Google Scholar
  15. Kuc, J., Henze, R.E., Ullstrup, A.J., Quackenbush, F.W.: Chlorogenic and caffeic acids as fungistatic agent produced by potatoes in response to inoculation withHelminthosporium carbonum.—J. amer. chem. Soc.78: 3123–3125, 1956.CrossRefGoogle Scholar
  16. Kumar, A., Pundhir, V.S., Gupta, K.C.: The role of phenols in potato tuber resistance against soft rot byErwinia carotovora.—Potato Res.34: 9–16, 1991.CrossRefGoogle Scholar
  17. Lyon, G.K., McGill, F.M.: Inhibition of growth ofErwinia carotovora in vitro by phenolics.—Potato Res.31: 461–467, 1988.CrossRefGoogle Scholar
  18. Metlitskii, L.V., Korableva, N.P., Sukhova, L.S.: Using Hydrel for minimizing sprouting of potato tubers during storage with concomitant reduction of losses due to pathogens.—Prikl. Biokhim. Mikrobiol.18: 111–119, 1982. [In Russ.]Google Scholar
  19. Röber, K.CH.: Investigation on the synthesis of polyphenols and phytoalexins in rot infected potato tubers.—Biochem. Physiol. Pflanz.184: 277–284, 1989.Google Scholar
  20. Rylski, I., Rappaport, L., Pratt, H.K.: Dual effects of ethylene on potato dormancy and sprout growth.—Plant Physiol.53: 658–662, 1974.PubMedCrossRefGoogle Scholar
  21. Shih, M., Kuc, J.: Incorporation of14C from acetate and mevalonate into rishitin and steroid glycoalkaliods by potato tuber slices inoculated withPhytophthora infestans.—Phytopathology63: 826–829, 1973.Google Scholar
  22. Vaughn, S.F., Lulai, E.: The involvement of mechanical barriers in the resistance response of a field-resistant and field-susceptible potato cultivar toVerticilium dahliae.—Physiol. mol. Plant Pathol.38: 455–465, 1991.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR 1995

Authors and Affiliations

  • M. Cvikrová
    • 1
  • J. Eder
    • 1
  • L. S. Sukhova
    • 2
  • N. P. Korableva
    • 2
  1. 1.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Institute of BiochemistryRussian Academy of SciencesMoscowRussia

Personalised recommendations