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Journal of Tongji Medical University

, Volume 16, Issue 3, pp 134–138 | Cite as

Investigation on the purification and expression of cell I — Hep I recombinant FN polypeptide inE. Coli

  • Zhang Guimei
  • Feng Zuohua
  • Li Dong
  • Zhang Hui
Article
  • 24 Downloads

Summary

An anti-metastatic polypeptide, bifunctional-domain (Cell I -Hep I) recombinant polypeptide of human fibronectin, was expressedin E. coli and purified. The expression level was found to be about 20%– 30 % of the total cell proteins. In BL21 (DE3)/T7, anE. coli expressing system, lactose can be used as an inducer to substitute IPTG thereby reducing the cost by several hundredfold and it is suitable for the large-scale preparation of recombinant FN polypeptide. Cell I -Hep 1 fragment is an alkaline polypeptide. In BL21(DE3)/T7 expressing system, better isolation was achieved if DEAE-52, instead of CM-52, was used for ion-exchange chromatography. The purified product was obtained after heparin-agarose affinity chromatography following ion-exchange chromatography.

Key words

fibronectin recombinant polypeptide induced expression purification metastasis 

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Copyright information

© Tongji Medical University 1996

Authors and Affiliations

  • Zhang Guimei
    • 1
  • Feng Zuohua
    • 1
  • Li Dong
    • 1
  • Zhang Hui
    • 1
  1. 1.Department of Medical Molecular BiologyTongji Medical UniversityWuhan

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