Abstract
The expression and regulation of metalloproteinases-2, -9 (MMP-2, -9) and their tissue inhibitors TIMP-1,-2, -3 mRNA were studied in this experiment. In the PMSG-hCG primed pseudopregnant rat, MMP-2, -9 mRNA levels were the highest at Day 1, decreased from Day 4, and reached the minimal level at Day 8, then increased at Day 14; no significant changes were observed in TIMP-2 mRNA expression from Day 1 to Day 14; TIMP-3 mRNA expression was the lowest at Day 1, increased from Day 4, reached the maximal level at Day 8, and persisted to Day 14. TNF-α could significantly increase the expression of MMP-2, -9 and TIMP-1 mRNA in thein vitro perfused pseudopregnant CL, and decrease the expression of TIMP-3 mRNA, but had no effect on TIMP-2 mRNA expression. The results indicate that MMP-2, -9 and TIMP-1, -2, -3 might be involved in the regulation of CL function and maintenance of CL structure via their coordinated gene expression. TNF-a could inhibit luteal regression via increasing MMP-2, -9 and TIMP-1 mRNA in thein vitro perfused pseudopregnant ovary.
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Harris, E. D., Welgus, H. G., Krane, S. M., Regulation of the mammalian collagen, Relat. Res., 1984, 4: 493.
Murdoch, W. J., Steadman, L. E., Balden, E. L., Immunoregulation of luteolysis, Med. Hypotheses, 1988, 27: 197.
Endo, T., Aten, R. F., Wang, F., Coordinate induction and activation of metalloproteinases and ascorbate depletion in structural lutelysis, Endocrinology, 1993, 133: 690.
Hulboy, D. L., Rudolph, L. A., Matrisian, L. M., Matrix metalloproteinases as mediators of reproductive function, Mol. Hum. Reprod., 1997, 3: 27.
Stricklin, G., Li, L., Jancie, V. et al., Localization of mRNA representing collagenase and TIMP in sections of healing human burn wounds, Am. J. Pathol., 1993, 143: 1657.
Harris, E. Jr., Mainardi, C., Pathobiochemistry, CRC Press, 1992, 199.
Liotta, L. A., Stetler-Stevenson, W. G., Metalloproteinases and cancer invasion, Semin. Cancer Biol., 1990, 1: 99.
Ji, I., Slaughter, R. G., Ellis, J. A. et al., Analysis of ovine corpora lutea for tumor necrosis factor mRNA and bioactivity during prostaglandin-induced luteolysis, Mol. Cell Endocrinol., 1991, 81: 77.
Show, D. W., Britt, J. H., Concentrations of tumor necrosis factor-a and progesterone within the bovine corpus luteum sampled by continuous flow microdialysis during luteolysisin vivo, Biol. Reprod., 1995, 53: 847.
Sato, T., Ito, A., Ogata, Y. et al., Tumor necrosis factor alpha induces pro-matrix metalloproteinase-9 production in human uterine cervical fibroblasts but interleukin-1 alpha antagonizes the inductive effect of TNF alpha, FEBS Lett., 1996, 392: 175.
Luo, W. X., Zhu, C., Expression and regulation of mRNAs for insulin-like growth factor (IGF-I), IGF-binding protein-2, and LH receptor in the process of follicular atresia, Science in China, Ser. C., 2000, 43(3): 272.
Cunry, T. E., Jr., Mann, J. S., Huang, M. H. et al., Gelatinase and proteoglycanase during the periovulatory period in the rat, Biol. Reprod., 1992, 46: 256.
Stamouli, A., O’Sillivan, M. J., Thomas, E. J. et al., Suppression of matrix metalloproteinases production by hCG in cultures of human luteinized granulosa cells as a model for gonadotrophin-induced luteal rescue, J. Reprod. Fertil., 1996, 107: 235.
Warren, B., Samuel, C., Thomas, B. et al., Collagenase, gelatinase, and proteoglycanase ribonucleic acid expression and activity during luteal development, maintenance, and regression in the pseudopregnant rat ovary, Biol. Reprod., 1996, 54: 616.
Smith, G. W., Goetz, T. L., Anthony, R. V., Molecular cloning of an ovine ovarian tissue inhibitor of metalloproteinases: Ontogeny of messenger ribonucleic acid expression andin situ localization within preovulatory follicles and luteal tissue, Endocrinology, 1994, 134: 344.
Pitzel, L., Jarry, H., Wuttke, W., Effects and interactions of prostaglandin F2a, oxytocin, and cytokines on steroidogenesis of porcine luteal cells, Endocrinol., 1993, 132: 751.
Benyo, D. F., Pate, J. L., Tumor necrosis factor-a alters bovine luteal cell synthetic capacity and viability, Endocrinol., 1992, 130: 854.
Pate, J., Involvement of immune cell in regulation of ovarian function, J. Reprod. Fetil. Suppl., 1995, 49: 365.
Juengel, J. L., Smith, G. W., Smith, M. F., Pattern of protein production by bovine corpora lutea during luteolysis and characterization of expression of two major secretory products of the regressing corpora lutea, J. reprod. Fertil., 1994, 100: 515.
Pescador, N., Soumano, K., Stocco, T. M. et al., Steroidogenic acute regulatory protein in bovine corpora lutea, Biol. Reprod., 1996, 55: 485.
Sandhoff, T. W., Mclean, M. P., Prostaglandin F2 a reduces steroidogenic acute regulatory (StAR) protein messenger ribonucleic acid expression in rat ovary, Endocrine, 1996, 5: 259.
Adashi, E. Y., The potential relevance of cytokines to ovarian physiology: The emerging role of resident ovarian cells of the white blood series, Endocr. Rev., 1990, 11: 454.
Wang, H. Z., Sheng, W. X., Liu, S. H. et al., Inhibitory effect of interferon and tumor necrosis factor on human luteal function in vitro, Fertil. Steril., 1992, 58: 941.
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Yu, X., Li, Q., Wang, H. et al. Expression and regulation of metalloproteinases-2, -9 and tissue inhibitors of metalloproteinases in rat corpus luteum. Chin. Sci. Bull. 47, 1011–1014 (2002). https://doi.org/10.1007/BF02907572
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DOI: https://doi.org/10.1007/BF02907572