Molecular Biotechnology

, Volume 4, Issue 1, pp 100–101 | Cite as

A new method of bipolymerase sequencing prevents “stop-bands”

  • Christopher C. Austin
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This article presents a relatively quick and cost-effective DNA sequencing method that prevents the formation of stop-bands. This method uses a combination ofTaq and Sequenase that allows sequencing at both low and high temperatures. The ability to sequence at a high temperature appears to be the fundamental component in preventing stop-band formation in G + C rich regions.

Index Entries

Artifactual DNA polymerase Taq Sequenase 


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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Christopher C. Austin
    • 1
  1. 1.Department of ZoologyUniversity of TexasAustin

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