A new method of bipolymerase sequencing prevents “stop-bands”
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This article presents a relatively quick and cost-effective DNA sequencing method that prevents the formation of stop-bands. This method uses a combination ofTaq and Sequenase that allows sequencing at both low and high temperatures. The ability to sequence at a high temperature appears to be the fundamental component in preventing stop-band formation in G + C rich regions.
Index EntriesArtifactual DNA polymerase Taq Sequenase