Biologia Plantarum

, Volume 31, Issue 2, pp 121–125 | Cite as

Regeneration responses of callus from different expiants and changes in isozymes during morphogenesis in wheat

  • H. S. Chawla


Immature embryo and root meristem expiants of wheat were cultured on modified medium of Murashige and Skoog and Gamborg’s medium supplemented with 2,4-dichlorophenoxy acetic acid. Morphogeriic callus cultures were obtained from both the expiants. The frequency of shoot formation varied from 22% to 48% from callus obtained from embryos while only root formation could be induced from root meristem expiants. Cultures from young and old non-differentiating calli, and calli with shoot and/or root formation at different intervals were analysed for isozymes of esterase, peroxidase and acid phosphatase for studying the morphogenic capacity. With the development of shoot and/or root from callus, some conspicuous isozymes appeared which indicates the involvement of these isozymes in root/shoot development rather than in the induction of morphogenesis in callus. Basic isozyme pattern of each enzyme for the callus was retained in all the callus stages.


Callus Culture Shoot Formation Immature Embryo Root Meristem Naphthalene Acetic Acid 
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Copyright information

© Academia 1989

Authors and Affiliations

  • H. S. Chawla
    • 1
  1. 1.Plant Breeding DepartmentG. B. Pant UniversityPantnagar, U. P.India

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