Biologia Plantarum

, 31:81 | Cite as

Polyamine metabolism in hants : Arginine and omithine decarboxylase activity in ripeningTrlticum durum seeds

  • M. Cecilia Anguillesi
  • Isa Grilli
  • C Floris


The pattern of the activity of arginine decarboxylase (ADC) and omithine decarboxylase (ODC) involved in polyamine synthesis in ripening wheat seeds was examined. The aim was to study the polyamines and the activity of the two enzymes in correlation with the growth processes occurring in the developing wheat seeds. The results obtained showed a very different pattern of polyamine content in the two organs of caryopsis, and that the two enzymes in the embryos have a higher activity than in the endosperms. Moreover, while in the embryos the ADC exhibits higher activity than the ODC, in the endosperms the activity of ODC is about similar to that of ADC. This pattern is discussed in relation to the different histological characteristics of embryo and endosperm tissues during seed development.

Additional index words

Embryo endosperm 


  1. Abraham, A. K., Pihl, A.: Role of polyamines in macromolecular synthesis.Trends biochem. Sci.6:106–107,1981.CrossRefGoogle Scholar
  2. Altman, A., Friedman, R., Levin, N.: Arginine and ornithine decarboxylase, the polyamine biosynthetic enzymes of mung bean seedlings.Plant. Physiol.69:876–879. 1982.PubMedGoogle Scholar
  3. Anguillesi, M. C., Floris, C., Grilli, I., Meletti, P.: Metabolism in wheat seed dormancy : polyamine content during early germination.Biochem. Biophys. Pflanzen173 :340–346, 1978.Google Scholar
  4. Anguillesi, M. C., Grilli, I., Floris, C.: Rate of synthesis of spermine and spermidine in germinating seeds ofGlycine, Helianthus and Triticum. Planta148:24–27, 1980.CrossRefGoogle Scholar
  5. Anguillesi, M. C., Grilli, I., Floris, C.: Polyamines and protein metabolism in maize inbreds differing in seed protein content.J. exp. Bot.33:1014–1020, 1982.CrossRefGoogle Scholar
  6. Bachrach, U.: Function of Naturally Occurring Polyamines.Acad. Press, New York 1973.Google Scholar
  7. Bagni, N.: [Spermine and spermidine in seeds.] In Ital.Giorn. Bot. Ital.102 :67–72,1968.Google Scholar
  8. Bagni, N., Serafini Fracassini, D., Torrigiani, P.: Polyamines and growth in higher plants. - In:Caldarera, C. M., Zappia, V., Bachrach, U.: Advances in Polyamines Research. Vol. 3. Pp. 377–387. Raven Press, New York 1981.Google Scholar
  9. Bensadoun, A., Weinstein, D.: Assay of proteins in the presence of interfering materials.Anal. Biochem.70: 241–250,1976.PubMedCrossRefGoogle Scholar
  10. Cohen, E., Heimer, Y. M., Mizrahi, Y.: Ornithine decarboxylase and arginine decarboxylase activities in meristematic tissue of tomato and potato plants.Plant Physiol.70 :544–546,1982a.PubMedGoogle Scholar
  11. Cohen, E., (MAlis)Arad, S., Mizrahi, Y.: Participation of ornithine decarboxylase in early stages of tomato fruit development.Plant Physiol.70 : 540–543,1982b.PubMedGoogle Scholar
  12. Copole, E. W., Fulungton, J. C., Kasarda, D. D.: Grain protein variability among species of Triticum andAegilops: quantitative SDS-PAGE studies.Theor. appl. Genet.60 :17–30,1981.CrossRefGoogle Scholar
  13. Dai, Y. R., Kaur-Sawhnby, R., Galston, A. W.: Promotion by gibberellic acid of polyamine biosynthesis in internodes of light-grown dwarf peas.Plant Physiol.69:103–105,1982.PubMedGoogle Scholar
  14. Galston, A. W.: Polyamines as modulators of plant development.Bioscience33 :382–388,1983.CrossRefGoogle Scholar
  15. Grilli, I., Anguillesi, M. C., Floris, C.: Protein and RNA content and synthesis in embryos and endosperms from developingTriticum durum seeds.Biol. Plant.31:8–18,1989.CrossRefGoogle Scholar
  16. Heimer, Y. M., Mizrahi, Y., Bachrach, U.: Ornithine decarboxylase activity in rapidly proliferating plant cells.FEBS Lett.104 :146–148,1979.PubMedCrossRefGoogle Scholar
  17. Heimer, Y. M., Mizrahi, Y.: Characterization of ornithine decarboxylase of tobacco cells and tomato ovaries.Biochem. J.201:373–376,1982.PubMedGoogle Scholar
  18. Lin, P. P. C.: Polyamine metabolism and its relation to response of the aleurone layers of barley seeds to gibberellic add. Plant Physiol.74 :975–983,1984.PubMedCrossRefGoogle Scholar
  19. Palavan, N., Galston, A. W.: Polyamine biosynthesis and titer during various developmental stages ofPhaseolus vulgaris. Physiol. Plant.55 :438–444,1982.CrossRefGoogle Scholar
  20. Pala van, N., Goren, R., Galston, A. W.: Effects of some growth regulators on polyamine biosynthetic enzymes in etiolated pea seedlings.Plant Cell Physiol.25 :541–546,1984.Google Scholar
  21. Peterson, R. F.: Wheat: botany, cultivation and utilization.Polunin, N. (ed.) : Leonard Hill Books, London 1965.Google Scholar
  22. Sen, K., Choudhuri, M. M., Ghosh, B.: Changes in polyamine contents during development and germination of rice seeds.Phytochemistry20:631–633,1981.CrossRefGoogle Scholar

Copyright information

© Academia 1989

Authors and Affiliations

  • M. Cecilia Anguillesi
    • 1
  • Isa Grilli
    • 1
  • C Floris
    • 1
  1. 1.Department of Plant SciencesPlant Physiology LaboratoryPisaItaly

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