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Plant Molecular Biology Reporter

, Volume 11, Issue 4, pp 317–319 | Cite as

A rapid and efficient method for the extraction of total DNA from mature leaves of the data palm (Phoenix dactylifera L.)

  • Mustapha Aitchitt
  • Charles C. Ainsworth
  • Madan Thangavelu
Commentary

Abstract

A method is presented for the rapid isolation of high-molecular-weight DNA from mature leaves of date palm (Phoenix dactylifera L.), using a CTAB-based buffer. The method yields up to 800 μg of DNA from 1 g of leaf tissues. The procedure was also suitable for DNA extraction from callus or buds from tissue culture. The DNA obtained through this method was a good substrate for at least seventeen restriction endonucleases. This method was also used to extract DNA from mature leaves of coconut and may be applicable to other species of palms.

Key Words

date palm Phoenix dactyliferaDNA extraction CTAB precipitation 

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Copyright information

© Springer 1993

Authors and Affiliations

  • Mustapha Aitchitt
    • 1
  • Charles C. Ainsworth
    • 1
  • Madan Thangavelu
    • 1
  1. 1.Department of Biochemistry and Biological SciencesWye College University of LondonAshfordEngland

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