Ricerca in clinica e in laboratorio

, Volume 13, Issue 2, pp 221–227 | Cite as

Measurement of low levels of immunoglobulins

A simple solid-phase radioimmunoassay
  • Claudio Carini
  • Jonathan Brostoff
Original Contributions


A solid-phase radioimmunoassay for the measurement of immunoglobulins is described. which is simple to perform and possesses sensitivity down to 1 ng/ml. Using anti-light chain serum in the solid-phase, antibody of any class can be measured using suitable labelled antisera.


Antibodies Antisera IgE IgG IgM Immunoglobulins Radioimmunoassay 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Brostoff J., Carini C., Wraith D. G., Johns P.: Production of IgE complexes by allergen challenge in atopic patients and the effect of sodium chromoglycate—Lancetii, 1268, 1979.CrossRefGoogle Scholar
  2. 2.
    Fraker P. J., Sperck J. C.: Protein and cell membrane iodinations with a sparingly soluble chloromide 1,2,3,4,6-tetrachloro-3a,6a-diphenylglycouril.—Biochem. biophys. Res. Commun.80, 849, 1978.PubMedCrossRefGoogle Scholar
  3. 3.
    Johns P., Stanworth D. R.: A simple numerical method for the construction of isokinetic sucrose density gradients and their application to the characterization of immunoglobulin complexes—J. immunol. Meth.10, 231, 1976.CrossRefGoogle Scholar
  4. 4.
    Lydyard P. M., Hayward A. R.: Induction of suppression through human T cell interaction—Clin. exp. Immunol.39, 486, 1980.Google Scholar
  5. 5.
    Romagnani S., Del Prete G. F., Giudizi G. M., Almericogna F., Ricci M.: A simple solid-phase radioimmunoassay for the measurement of IgG, secretedin vitro by human lymphocytes. —J. immunol. Meth.29, 263, 1979.CrossRefGoogle Scholar
  6. 6.
    Waldman T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W.: Role of suppressor T cells in the pathogenesis of common hypoglobulinaemia—Lancetii, 609, 1974.CrossRefGoogle Scholar
  7. 7.
    Wide L.: Radioimmunoassay employing immunosorbents—Acta endocr. (Kbh.)63 (Suppl. 142), 207, 1969.Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Claudio Carini
    • 1
  • Jonathan Brostoff
    • 1
  1. 1.Department of ImmunologyThe Middlesex Hospital Medical SchoolLondon

Personalised recommendations