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Effects of nitric oxide on proliferation and apoptosis of cultured bovine trabecular meshwork cells

  • Xue Wei
  • Du Shuhua
  • Li Yong
  • Yang Yejing
  • Sun Jinghua
Article

Summary

The effects of different doses of nitric oxide (NO) on the proliferation and apoptosis of the cultured bovine trabecular meshwork (TM) cells were studied. L-arginine and NG-nitro-L-arginine methyl (L-NAME) were incubated with TM cells for 48h. In the control group, no medicine was given. In the experimental groups, concentrations of L-arginine and L-NAME were 1×10−7 mol/L, 1×10−6 mol/L, 1×10−5 mol/L, 1×10−4 mol/L, 1×10−3 mol/L and 1×10−2 mol/L, respectively. NO 2 in supernate, the proliferation and apoptosis of TM cells and mRNA expression of bcl-2 and bax were measured by Griess reagent, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), MTT assay and in situ hybridization, respectively. The results showed that L-arginine with concentration ≥1×10−4 mol/L could induce apoptosis of the TM cells and inhibit the proliferation of TM cells through increasing the NO levels, down-regulating bcl-2 mRNA expression and up-regulating bax mRNA expression; L-NAME with concentration ≥1×10−5 mol/L could induce the proliferation of the TM cells through suppressing the production of NO. It was concluded that NO in high level could induce apoptosis of the TM cells and suppress the proliferation of the TM cells.

Key words

nitric oxide trabecular meshwork cells proliferation apoptosis 

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Copyright information

© Springer 2002

Authors and Affiliations

  • Xue Wei
    • 1
  • Du Shuhua
    • 1
  • Li Yong
    • 2
  • Yang Yejing
    • 3
  • Sun Jinghua
    • 1
  1. 1.Department of Ophthalmology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan
  2. 2.Department of General Surgery, Xiehe Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan
  3. 3.Department of Molecular Biology, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

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