Summary
In order to investigate the possibility of expression of exogenous gene in transduced articular chondrocytes, plasmid pcDNA3-rhBMP7 was delivered to cultured chondrocytes. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected. And the bioactivity of transgene expression product was detected through MTT assay as well. It was confirmed that exogenous gene could be expressed efficiently in transduced chondrocytes and the transgene therapy on the problems of articular cartilge.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Grande D A, Breibart A S, Mason J Met al. Cartilage tissue engineering: current limitations and solutions. Clin Orthop, 1999, 367S: 176
Cook S D, Rueger D C. Osteogenic protein-1: biology and application. Clin Orthop, 1996, 324: 29
Goldring M B. Skeletal tissue response cytokines. Clin Orthop, 1990, 258: 245
Evans C H Robbins P D. Current concepts review. Possible orthopaedic applications of gene therapy. J Bone and Joint Surg, 1995, 77A: 1103
Author information
Authors and Affiliations
Additional information
DUAN Deyu, male, born in 1971, Doctor in Charge
Rights and permissions
About this article
Cite this article
Deyu, D., Jingyuan, D., Yong, L. et al. Transfection of articular chondrocytes with rhBMP7 gene and its expression. Current Medical Science 22, 42–43 (2002). https://doi.org/10.1007/BF02904785
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02904785