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Transfection of articular chondrocytes with rhBMP7 gene and its expression

  • Duan Deyu
  • Du Jingyuan
  • Liu Yong
  • Guo Xiaodong
  • Wang Hong
Article
  • 19 Downloads

Summary

In order to investigate the possibility of expression of exogenous gene in transduced articular chondrocytes, plasmid pcDNA3-rhBMP7 was delivered to cultured chondrocytes. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected. And the bioactivity of transgene expression product was detected through MTT assay as well. It was confirmed that exogenous gene could be expressed efficiently in transduced chondrocytes and the transgene therapy on the problems of articular cartilge.

Key words

transfection articular chondrocytes gene bone morphogenetic protein-7 

References

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Copyright information

© Springer 2002

Authors and Affiliations

  • Duan Deyu
    • 1
  • Du Jingyuan
    • 1
  • Liu Yong
    • 1
  • Guo Xiaodong
    • 1
  • Wang Hong
    • 1
  1. 1.Department of Orthopedics, Xiehe Hospital, Tongji medical CollegeHuazhong University of Science and TechnologyWuhan

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