Virological, neurological and histological investigations of a child born to a mother with AIDS
HIV-1 was isolated from a child at 6 and 9 months of age, proving the vertical transmission of infection from the mother with AIDS. The p24 antigen test of the plasma at 9 months of age was positive as well. A positive PCR reaction was detected in J34 cells, infected with the supernatant of the peripheral blood lymphocytes of the child. According to phenotypic characterization, the virus proved to be a SI (syncytium inducing) isolate, growing in PBL, MT2, J34 and other T and monocytic cell lines. The isolate was AZT sensitive. Two methods were applied for genotypic characterization: 1. Heteroduplex mobility assay (HMA), 2. Sequence analysis of a part of the env gene. On the basis of both of these methods, this virus belongs to the B subtype of HIV-1, which is prevalent mainly in Europe and in the USA. The neurological status of the child was followed regularly. At autopsy the presence of p24 antigen was detected in glial cells of the frontal cortex, proving the presence of the virus in the brain. A retardation of the development of the central nervous system could be observed as well.
Key wordsHIV mother to child transmission central nervous system development delayed myelinization
Unable to display preview. Download preview PDF.
- 2.Burgard M, Mayax MJ, Blanche S, et al.: The use of viral culture and p24 antigen testing to diagnose human immunodeficiency virus infection in neonates. New Engl J Medicine 327:1192–1197, 1992.Google Scholar
- 3.Caputo A, Sodroski JG, Haseltine WA: Constitutive expression of HIV-1 tat protein in human Jurkat T cells using a BK virus vector. J AIDS 3:372–379, 1990.Google Scholar
- 6.Delwart EL, Herring B, Rodrigo AG, Mullins JI: Genetic subtyping of human immunodeficiency virus using a heteroduplex mobility assay. PCR Meth Appl 4:202–216, 1995.Google Scholar
- 8.Gardner S and Popovic M: Virus isolation and production Techniques in HIV research 53–70, Stockton Press 1990.Google Scholar
- 9.Gyuris Á, Földes I, Jobbágy A: A self made immunofluorescence assay for supplementing other confirmatory methods of HIV-antibodies. Z.Klin.Med. 45:339–340, 1990.Google Scholar
- 11.Levy JA: HIV and the Pathogenesis of AIDS, ASM Press. Washington, D.C. 1994.Google Scholar
- 12.Mitsuya H, Weinhold KJ, Furman PA et al.:3′-Azido-3- deoxythymidine (BW A509U): An antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro. Proc Natl Acad Sci USA 82:7096–7100, 1985.PubMedCrossRefGoogle Scholar