In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence
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A method using synchrotron radiation X-ray fluorescence analysis toin situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak III in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique forin situ analysis of trace elements in protein bands.
Keywordssynchrotron radiation X-ray fluorescence analysis in situ analysis metalloprotein, electrophoreticseparation
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