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In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

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Abstract

A method using synchrotron radiation X-ray fluorescence analysis toin situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak III in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique forin situ analysis of trace elements in protein bands.

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Authors and Affiliations

  1. Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, 100080, Beijing, China

    Chunying Chen, Peiqun Zhang & Zhifang Chai

  2. Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, 100080, Beijing, China

    Guangcheng Li & Yuying Huang

Authors
  1. Chunying Chen
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  2. Peiqun Zhang
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  3. Zhifang Chai
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  4. Guangcheng Li
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  5. Yuying Huang
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Correspondence to Chunying Chen.

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Chen, C., Zhang, P., Chai, Z. et al. In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence. Sci. China Ser. A-Math. 43, 88–92 (2000). https://doi.org/10.1007/BF02903851

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  • DOI: https://doi.org/10.1007/BF02903851

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