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In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

  • Chunying Chen
  • Peiqun  Zhang
  • Zhifang Chai
  • Guangcheng Li
  • Yuying Huang
Article

Abstract

A method using synchrotron radiation X-ray fluorescence analysis toin situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak III in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique forin situ analysis of trace elements in protein bands.

Keywords

synchrotron radiation X-ray fluorescence analysis in situ analysis metalloprotein, electrophoreticseparation 

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Copyright information

© Science in China Press 2000

Authors and Affiliations

  • Chunying Chen
    • 1
  • Peiqun  Zhang
    • 1
  • Zhifang Chai
    • 1
  • Guangcheng Li
    • 2
  • Yuying Huang
    • 2
  1. 1.Laboratory of Nuclear Analytical TechniquesInstitute of High Energy Physics, Chinese Academy of SciencesBeijingChina
  2. 2.Synchrotron Radiation LaboratoryInstitute of High Energy Physics, Chinese Academy of SciencesBeijingChina

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