Biologia Plantarum

, Volume 25, Issue 6, pp 456–461 | Cite as

Comparison of malate dehydrogenase isoenzymes in someAllium species by isoelectric focusing

  • Vera Hadacová
  • Eva Klozová
  • Jiŕina Svachulová
  • Kveta Pitterová
Original Papers


Malate dehydrogenase isoenzymes were studied in tenAllium species and in six cultivars ofA. cepa by isoelectric focusing in polyacrylamide gel with Ampholine pH 3.5–10.0. Using this method better resolution was obtained than by polyacrylamide gel electrophoresis. The number of MDH isoenzymes obtained by isoelectric focusing is from five to ten in the range of pH 3.65 to 6.75. MDH isoenzymes can be used for characterization on the level of species and cultivars (inA. cepa), but its use on the level of sections and subgenera is questionable.


Malate Dehydrogenase Seed Protein Jaccard Index Isoenzyme Pattern Allium Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Denford, K. E., Vaughan, J. G.: A comparative study of certain seed isoenzymes in the ten chromosome complex ofBrassica campestris and its allies. - Ann. Bot.41: 411–418, 1977.Google Scholar
  2. Grimwood, B. G., McDaniel, R. G.: Variant malate dehydrogenase isozymes in mitochondrial populations. -Biochim. Biophys. Acta220: 410–415, 1970.PubMedGoogle Scholar
  3. Hadacová, V., Klozová, E., Hadac, E., Turková, V., Pitterová, K.: Comparison of esterase isoenzyme patterns in seeds of someAllium species and in cultivars ofAllium cepa L. - Biol. Plant.23: 174–181, 1981a.CrossRefGoogle Scholar
  4. Hadacová, V., Klozová, E., Pitterová, K., Turková, V.: The screening of the enzyme and isoenzyme patterns in seeds ofAllium cepa L. - Biol. Plant.23: 442–448, 1981b.CrossRefGoogle Scholar
  5. Hadacová, V., Svachulová, J., Klozová, E., Hadac, E., Pitterová, K.: Use of esterase isoenzymes revealed by gel isoelectric focusing as an aid in chemotaxonomical study of the genusAllium.- Biol. Plant.25: 36–42, 1983.CrossRefGoogle Scholar
  6. Hadacová, V., Turková, V., Hadac, E., Klozová, E.: Comparison of seed proteins of some representatives of the genusPisum from the point of view of their relationship. Comparison by disc electrophoresis. -Biol. Plant.22: 7–16, 1980.CrossRefGoogle Scholar
  7. Johnson, B. L., Hall, O.: Analysis of phylogenetic affinities in theTriticinae by protein electrophoresis. -Amer. J. Bot.52: 506–513, 1965.CrossRefGoogle Scholar
  8. Klozová, V., Turková, V., Hadacová, V., Svachulová, J.: Serological comparisons of seed proteins of someAllium (L.) species belonging to the subgenusRhizirideum (G. Don ex Koch) Wendelbo.- Biol. Plant.23: 376–380, 1981b.CrossRefGoogle Scholar
  9. Klozová, E., Turková, V., Pitterová, K., Hadacová, V.: Serological comparisons of seed proteins of some representatives of the genusAllium.- Biol. Plant.23: 9–15, 1981a.CrossRefGoogle Scholar
  10. Kuhns, L. J., Fretz, T. A.: Distinguishing rose cultivars by polyacrylamide gel electrophoresis. II. Isoenzyme variation among cultivars. - J. amer. Soc. hort. Sci.103: 509–516, 1978.Google Scholar
  11. McMillin, D. E., Scandalios, J. G.: Genetic, immunological, and gene dosage studies of mitochondrial and cytosolic MDH variants in maize. - J. Hered.73: 177–182, 1982.Google Scholar
  12. Nucca, R., Soave, C., Motto, M., Salamini, F.: Taxonomic significance of the zein isoelectric focusing pattern. - Maydica23: 239–249, 1978.Google Scholar
  13. Payne, R. C., Fairbrothers, D. E.: Disc electrophoretic evidence for heterozygosity and phenotypic plasticity in selected lines ofCoffea arabica L. - Bot. Gaz.137: 1–6, 1976.CrossRefGoogle Scholar
  14. Radman, M.: An endonuclease fromEscherichia coli that introduces single polynucleotide chain scissions in ultraviolet irradiated DNA. - J. biol. Chem.251: 1438–1445, 1976.PubMedGoogle Scholar
  15. Schechter, Y., De Wet, J. M. J.: Comparative electrophoresis and isozyme analysis of seed proteins from cultivated races ofSorghum.- Amer. J. Bot.62: 254–261, 1975.CrossRefGoogle Scholar
  16. Schrauwen, J.: Detection of enzymes after electrophoretic separation in polyacrylamide gel columns. - J. Chromatogr.23: 177–180, 1966.CrossRefGoogle Scholar
  17. Thurman, D. A., Boulter, D., Derbyshire, E., Turner, B. L.: Electrophoretic mobilities of formic and glutamic dehydrogenases in theFabaceae: A systematic survey. - New. Phytol.66: 37–45, 1967.CrossRefGoogle Scholar
  18. Webb, A. C., Ingalls, H. M.: Analysis of malate dehydrogenase isoenzymes from anuran amphibian ovary by isoelectric focusing. - Biochem. Genet.18: 1185–1205, 1980.PubMedCrossRefGoogle Scholar

Copyright information

© Academia 1983

Authors and Affiliations

  • Vera Hadacová
    • 1
  • Eva Klozová
    • 2
  • Jiŕina Svachulová
    • 2
  • Kveta Pitterová
    • 2
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6Czechoslovakia
  2. 2.Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6Czechoslovakia

Personalised recommendations