Biologia Plantarum

, Volume 25, Issue 4, pp 299–304 | Cite as

Similar degree of methyl methanesulphonate-induced DNA damage in two clones ofTradescantia differing in mutagenic sensitivity to alkylating agents

  • J. Velemínský
  • T. Gichner
  • V. Pokorný
  • J. Šatava
Original Papers


In two clones ofTradescantia (4430 and 02) differing in the sensitivity to the mutagenic action of alkylating agents, equimolar doses of [14C] methyl methanesulphonate (MMS) elicited a similar degree of protein, RNA and DNA alkylation and a similar amount of DNA-7-methylguanine and DNA-3-methyladenine in cells of inflorescence. Moreover, in the same clones and tissues the same doses of nonlabelled MMS produced a similar amount of DNA single strand breaks and/or alkali labile sites as measured in alkaline sucrose gradients. None of the DNA lesions followed is therefore decisive for explanation of the different mutagenic sensitivity ofTradescantia clones.


Single Strand Break Mutagenic Sensitivity Maleic Hydrazide Ethyl Methanesulphonate Dimethylnitrosamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

AP sites

apurinic and apyrimidinic sites in DNA

[14C] MMS

(14C)-methyl methanesulphonate






ethyl methanesulphonate


(3H) labelled thymidine




N7 methylguanine


methyl methanesulphonate


DNA single strand breaks


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  1. Bannon, P., Verly, W.: Alkylation of phosphates and stability of phosphate triesters in DNA. - Europe. J. Biochem.31: 103–111, 1972CrossRefGoogle Scholar
  2. Gichner, T., Velemínský, J., Pokorný, V.: Somatic mutations induced by maleic hydrazide and its potassium and diethanolamine salts in theTradescantia mutation assay. - Mutat. Res.103: 289–293, 1982.PubMedCrossRefGoogle Scholar
  3. Gichner, T., Velemínský, J., Underbrink, A. G.: Induction of somatic mutations by the promutagen dimethylnitrosamine in hairs ofTradescantia stamen. - Mutat. Res.78: 381 to 384, 1980.Google Scholar
  4. Hoffmann, G. R.: Genetic effects of dimethyl sulfate, diethyl sulfate and related compounds. - Mutat. Bes.75: 63–129, 1980.Google Scholar
  5. Karran, P., Higgins, N. P., Strauss, B.: Intermediates in excision repair by human cells: Use of S1 nuclease and benzoylated naphtoylated cellulose to reveal single strand breaks. - Biochemistry16: 4483–4490, 1977.PubMedCrossRefGoogle Scholar
  6. Krieg, D. B., Van’t Hof, J., Schairer, L. A.: On the mechanisms underlying mutagen-induced pink sectors inTradescantia. (Abstract) - Genetics (Suppl.)91: 63 - 64, 1979.Google Scholar
  7. Kudirka, D. T., Van’t Hof, J.: G 2 arrest and differentiation in the petals ofTradescantia clone 4430.- Exp. Cell Res.130: 443–450, 1980.PubMedCrossRefGoogle Scholar
  8. Mabmur, J.: A procedure for isolation of deoxyribonucleic acid from microorganisms. - J. mol. Biol.3: 208–218, 1961.CrossRefGoogle Scholar
  9. Nauman, C. H., Klotz, P. J., Schairer, L. A.: Uptake of tritiated 1,2-dibromoethane byTradescantia floral tissues: relation to induced mutation frequency in stamen hair cells. - Environ. exp. Bot.19: 201–215, 1979.CrossRefGoogle Scholar
  10. Nauman, C. H., Sparrow, A. H., Schairer, L. A.: Comparative effects of ionizing radiation and two gaseous chemical mutagens on somatic mutation induction in one mutable and two nonmutable clones ofTradescantia. - Mutat. Res.38: 53 - 70, 1976.Google Scholar
  11. Riazuddin, S., Lindahl, T., Properties of 3-methyladenine-DNA glycosylase fromEscherichia coli. -Biochemistry17: 2110–2118, 1978.PubMedCrossRefGoogle Scholar
  12. Sparrow, A. H., Schairer, L. A., Villalobos-Pietrini, R.: Comparison of somatic mutations rates induced inTradescantia by chemical and physical mutagens. - Mutat. Res.26: 265 to 276, 1974.Google Scholar
  13. Steensgaard, J., Møller, N. P. H., Funding, L.: Rate-zonal centrifugation: Quantitative aspects. - In:Birnie, G. D., Rickwood, D. (ed.): Centrifugal Separations in Molecular and Cell Biology. Pp. 115–167. Butterworths, London 1978.Google Scholar
  14. Underbrink, A. G., Schairer, L. A., Sparrow, A. H.:Tradescantia stamen hairs: a radiobiological test system applicable to chemical mutagenesis. - In:Hollaender, A. (ed.): Chemical Mutagens. Principles and Methods for their Detection, Vol. 3. Pp. 171 - 207. Plenum Press, New York 1973.Google Scholar
  15. Van der Schans, G. P., Aten, J. B. T., Block, J.: Determination of molecular weight distribution of DNA by means of sedimentation in a sucrose gradients. - Anal. Biochem.32: 14–30, 1969.PubMedCrossRefGoogle Scholar
  16. Velemínský, J., Zadrazil, S., Pokorný, V., Gichner, T., Švachulová, J.: Repair of single strand breaks and fate of N-7-methylguanine in DNA during the recovery from genetical damage induced by N-methyl-N-nitrosourea in barley seeds. - Mutat. Res.17: 49–58, 1973.Google Scholar

Copyright information

© Academia 1983

Authors and Affiliations

  • J. Velemínský
    • 1
  • T. Gichner
    • 1
  • V. Pokorný
    • 1
  • J. Šatava
    • 1
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6Czechoslovakia

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