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Biologia Plantarum

, Volume 25, Issue 4, pp 299–304 | Cite as

Similar degree of methyl methanesulphonate-induced DNA damage in two clones ofTradescantia differing in mutagenic sensitivity to alkylating agents

  • J. Velemínský
  • T. Gichner
  • V. Pokorný
  • J. Šatava
Original Papers

Abstract

In two clones ofTradescantia (4430 and 02) differing in the sensitivity to the mutagenic action of alkylating agents, equimolar doses of [14C] methyl methanesulphonate (MMS) elicited a similar degree of protein, RNA and DNA alkylation and a similar amount of DNA-7-methylguanine and DNA-3-methyladenine in cells of inflorescence. Moreover, in the same clones and tissues the same doses of nonlabelled MMS produced a similar amount of DNA single strand breaks and/or alkali labile sites as measured in alkaline sucrose gradients. None of the DNA lesions followed is therefore decisive for explanation of the different mutagenic sensitivity ofTradescantia clones.

Keywords

Single Strand Break Mutagenic Sensitivity Maleic Hydrazide Ethyl Methanesulphonate Dimethylnitrosamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

AP sites

apurinic and apyrimidinic sites in DNA

[14C] MMS

(14C)-methyl methanesulphonate

DBE

1,2-dibromoethane

DMN

dimethylnitrosamine

EMS

ethyl methanesulphonate

3HTdR

(3H) labelled thymidine

3-MeA

N3-methyladenine

7-MeG

N7 methylguanine

MMS

methyl methanesulphonate

SB

DNA single strand breaks

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Copyright information

© Academia 1983

Authors and Affiliations

  • J. Velemínský
    • 1
  • T. Gichner
    • 1
  • V. Pokorný
    • 1
  • J. Šatava
    • 1
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6Czechoslovakia

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