Abstract
In two clones ofTradescantia (4430 and 02) differing in the sensitivity to the mutagenic action of alkylating agents, equimolar doses of [14C] methyl methanesulphonate (MMS) elicited a similar degree of protein, RNA and DNA alkylation and a similar amount of DNA-7-methylguanine and DNA-3-methyladenine in cells of inflorescence. Moreover, in the same clones and tissues the same doses of nonlabelled MMS produced a similar amount of DNA single strand breaks and/or alkali labile sites as measured in alkaline sucrose gradients. None of the DNA lesions followed is therefore decisive for explanation of the different mutagenic sensitivity ofTradescantia clones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AP sites:
-
apurinic and apyrimidinic sites in DNA
- [14C] MMS:
-
(14C)-methyl methanesulphonate
- DBE:
-
1,2-dibromoethane
- DMN:
-
dimethylnitrosamine
- EMS:
-
ethyl methanesulphonate
- 3HTdR:
-
(3H) labelled thymidine
- 3-MeA:
-
N3-methyladenine
- 7-MeG:
-
N7 methylguanine
- MMS:
-
methyl methanesulphonate
- SB:
-
DNA single strand breaks
References
Bannon, P., Verly, W.: Alkylation of phosphates and stability of phosphate triesters in DNA. - Europe. J. Biochem.31: 103–111, 1972
Gichner, T., Velemínský, J., Pokorný, V.: Somatic mutations induced by maleic hydrazide and its potassium and diethanolamine salts in theTradescantia mutation assay. - Mutat. Res.103: 289–293, 1982.
Gichner, T., Velemínský, J., Underbrink, A. G.: Induction of somatic mutations by the promutagen dimethylnitrosamine in hairs ofTradescantia stamen. - Mutat. Res.78: 381 to 384, 1980.
Hoffmann, G. R.: Genetic effects of dimethyl sulfate, diethyl sulfate and related compounds. - Mutat. Bes.75: 63–129, 1980.
Karran, P., Higgins, N. P., Strauss, B.: Intermediates in excision repair by human cells: Use of S1 nuclease and benzoylated naphtoylated cellulose to reveal single strand breaks. - Biochemistry16: 4483–4490, 1977.
Krieg, D. B., Van’t Hof, J., Schairer, L. A.: On the mechanisms underlying mutagen-induced pink sectors inTradescantia. (Abstract) - Genetics (Suppl.)91: 63 - 64, 1979.
Kudirka, D. T., Van’t Hof, J.: G 2 arrest and differentiation in the petals ofTradescantia clone 4430.- Exp. Cell Res.130: 443–450, 1980.
Mabmur, J.: A procedure for isolation of deoxyribonucleic acid from microorganisms. - J. mol. Biol.3: 208–218, 1961.
Nauman, C. H., Klotz, P. J., Schairer, L. A.: Uptake of tritiated 1,2-dibromoethane byTradescantia floral tissues: relation to induced mutation frequency in stamen hair cells. - Environ. exp. Bot.19: 201–215, 1979.
Nauman, C. H., Sparrow, A. H., Schairer, L. A.: Comparative effects of ionizing radiation and two gaseous chemical mutagens on somatic mutation induction in one mutable and two nonmutable clones ofTradescantia. - Mutat. Res.38: 53 - 70, 1976.
Riazuddin, S., Lindahl, T., Properties of 3-methyladenine-DNA glycosylase fromEscherichia coli. -Biochemistry17: 2110–2118, 1978.
Sparrow, A. H., Schairer, L. A., Villalobos-Pietrini, R.: Comparison of somatic mutations rates induced inTradescantia by chemical and physical mutagens. - Mutat. Res.26: 265 to 276, 1974.
Steensgaard, J., Møller, N. P. H., Funding, L.: Rate-zonal centrifugation: Quantitative aspects. - In:Birnie, G. D., Rickwood, D. (ed.): Centrifugal Separations in Molecular and Cell Biology. Pp. 115–167. Butterworths, London 1978.
Underbrink, A. G., Schairer, L. A., Sparrow, A. H.:Tradescantia stamen hairs: a radiobiological test system applicable to chemical mutagenesis. - In:Hollaender, A. (ed.): Chemical Mutagens. Principles and Methods for their Detection, Vol. 3. Pp. 171 - 207. Plenum Press, New York 1973.
Van der Schans, G. P., Aten, J. B. T., Block, J.: Determination of molecular weight distribution of DNA by means of sedimentation in a sucrose gradients. - Anal. Biochem.32: 14–30, 1969.
Velemínský, J., Zadrazil, S., Pokorný, V., Gichner, T., Švachulová, J.: Repair of single strand breaks and fate of N-7-methylguanine in DNA during the recovery from genetical damage induced by N-methyl-N-nitrosourea in barley seeds. - Mutat. Res.17: 49–58, 1973.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Velemínský, J., Gichner, T., Pokorný, V. et al. Similar degree of methyl methanesulphonate-induced DNA damage in two clones ofTradescantia differing in mutagenic sensitivity to alkylating agents. Biol Plant 25, 299–304 (1983). https://doi.org/10.1007/BF02902878
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02902878