Biologia Plantarum

, 28:270 | Cite as

Localization of acid phosphatase activity in maize root under phosphorus deficiency

  • Marie Kummerová
Original Papers


The effect of phosphorus deficiency on acid phosphatase activity in the apical, middle and basal parts of the root of maize plants was followed. The supernatant obtained by centrifuging the homogenate of plant tissue at 1500 ×g was further centrifuged at 18 000 ×g, the sediment being marked as fraction II and the supernatant as fraction III. The results obtained document the fact that acid phosphatase activity of the two fractions of all analyzed root segments was higher in plants cultured in nutrient medium without phosphate than in those containing phosphorus in nutrient medium. In most cases this difference was significant to highly significant. The results of experiments proved unambiguously a higher enzymatic activity in all root segments in fraction III than in fraction II. In fraction III the highest acid phosphatase activity was found in the apical part, in fraction II in the basal part of the root.


Acid Phosphatase Maize Root Root Segment Acid Phosphatase Activity Phosphorus Deficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Anderset, G., Greppin, H.: Mise en évidence de deux types d’activité phosphatatique acide dans le meristème. - Saussurea6: 259–269, 1975.Google Scholar
  2. Besford, K. T.: A rapid tissue test for diagnosing phosphorus deficiency in the tomato plant. - Ann. Bot.45: 225–227, 1980.Google Scholar
  3. Besford, R. T., Syred, A. D.: Effect cf phosphorus nutrition on the cellular distribution of acid phosphatase in theLycopersicon esculentum L., - Ann. Bot.43: 431–435, 1979.Google Scholar
  4. Bieleski, R. L.: Effect of phosphorus deficiency on levels of phosphorus compounds inSpirodela. - Plant Physiol.43: 1309–1316, 1968.PubMedGoogle Scholar
  5. Bieleski, R. L.: Phosphate pools, phosphate transport and phosphate availability. - Annu. Rev. Plant Physiol.24: 225–252, 1973.CrossRefGoogle Scholar
  6. Bieleski, R. L.: Development of an externally-located alkaline phosphatase as a response to phosphorus deficiency. - In:Bieleski, R. L., Ferguson, A. R., Cresswell, M. M.(ed.): Mechanisms of Regulation of Plant Growth. Pp. 165–170. Bull. 12 Roy, Soc. New Zeeland, Wellington 1974.Google Scholar
  7. Blum, J. J.: Observations on the acid phosphatase ofEuglena gracilis. - J. Cell Biol.24: 223 to 234, 1965.CrossRefGoogle Scholar
  8. Boutin, J. P., Provot, M., Roux, L.: Effect of cycloheximide and renewal of phosphorus supply on surface acid phosphatase activity on phosphorus deficient tomato roots. - Physiol. Plant.51: 353–360, 1981.CrossRefGoogle Scholar
  9. Clark, R. B.: Characterization of phosphatase of intact maize roots., - J. agr. Food Chem.23: 458–460, 1975.CrossRefGoogle Scholar
  10. Clarkson, D. T., Scattergood, Ch. B.: Growth and phosphate transport in barley and tomato plants during the development of, and recovery from, phosphate—stress. - J. exp. Bot.33: 865–875, 1982.CrossRefGoogle Scholar
  11. Emmert, F. H.: Comparison of centripetal passage of phosphorus across roots versus accumulation in individual cells, with respect to pH and ion supply. - J. Plant Nutrition6: 267–274, 1983.CrossRefGoogle Scholar
  12. Fiske, C. H., Subbarow, Y.: The colorimetric determination of phosphorus. - J. biol. Chem.66: 375–400, 1925.Google Scholar
  13. Kummerova, M.: The effect of phosphorus on activity of acid phosphatase in maize (Zea mays L.) - Scripta Fac. Sci. nat. Univ. Purk. Brun.13: 343–348, 1983.Google Scholar
  14. Kummerova, M., Buczek, J.: Nitrate reductase and acid phosphatase activities as affected by inorganic phosphate in corn roots. - Acta Soc. Bot. Pol.52: 77–86, 1983.Google Scholar
  15. Lachlan, K. D.: Acid phosphatase activity in intact roots and phosphorus nutrition in plants. I. Assay conditions and phosphatase activity. - Aust. J. agr. Res.31: 429–440, 1980.CrossRefGoogle Scholar
  16. Lux, A.: Ontogenesis of xylem cells in barley roots. II. Ultrastructural study of the acid phosphatase. - Acta Fac. Rer. nat. Univ. Comen., Physiol. Plant.18: 43–49, 1982.Google Scholar
  17. Press, M. C., Lee, J. A.: Acid phosphatase activity inSphagnum species in relation to phosphate nutrition.- New Phytol.93: 567–573, 1983.CrossRefGoogle Scholar
  18. Price, C. A.: Repression of acid phosphatase synthesis inEuglena gracilis. - Science135 : 46, 1962.PubMedCrossRefGoogle Scholar
  19. Rebeille, F., Bligny, R., Douce, R.: Regulation of Pi uptake byAcer pseudoplatanus cells.- Arch. Biochem. Biophys.219: 371–378, 1982.PubMedCrossRefGoogle Scholar
  20. Reid, M. S., Bieleski, R. L.: Changes in phosphatase activity in phosphorus-deficientSpirodela. - Planta94: 273–281, 1970.CrossRefGoogle Scholar

Copyright information

© Academia 1986

Authors and Affiliations

  • Marie Kummerová
    • 1
  1. 1.Department of Plant Biology, Faculty of ScienceUniversity of J. E. PurkyněBrnoCzechoslovakia

Personalised recommendations