Incorporation of [14C]-glutamate into proteins and chlorophylls inDunaliella tertiolecta, a marine chlorophyte
Incorporation of [14C]-glutamate into soluble proteins accounted for about 14% of the total glutamate uptake, while < 1% of the ammo acid carbon was incorporated into chlorophylla. Most of the remaining 85% of the glutamate was retained in low molecular mass pools. High uptake rates appeared to be related to a “shift-up” phenomenon, resulting from the addition of fresh media. Apparent turnover times, estimated from reverse isotope dilution experiments, varied from about 1 to 3.5 h for the soluble proteins and about 3 to 5 h for chlorophyll a. Rapid changes in glutamate to glutamine ratios, resulting from changes in irradiance, suggest that the intermediary metabolism of glutamate is regulated by irradiance.
KeywordsChlorophyll Glutamate Glutamine Synthetase Aminolevulinic Acid Marine Plankton Diatom
photosynthetically active radiation
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