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Chinese Science Bulletin

, Volume 46, Issue 23, pp 1980–1984 | Cite as

Expression ofBcl-2 in cells with different telomerase activities

  • Jing Chen
  • Jianguo Ren
  • Jianyu Zheng
  • Guoping Cai
  • Yaoren Dai
Notes

Abstract

Both telomerase andBcl-2 are important genes in controlling apoptosis. The activation of telomerase and the abnormal regulation ofBcl-2 are also closely related to carcinogenesis. However, little is known about the linkage between telomerase andBcl-2. The effect of activated telomerase on the expression ofBcl-2 has been investigated. It is demonstrated that in tumor and transformed cells with higher telomerase activity,Bcl-2 expression is significantly lower than that in telomerase negative or less telomerose activity cells. Further study showed that in the telomerase gene-transformed 2BS-fibroblasts,Bcl-2 expression is inhibited significantly while the exogenous telomerase catalytic subunit gene is re-expressed in fibroblasts. Results indicated that there might be a certain linkage between the expression of telomerase andBcl-2, and overexpression of exogenous telomerase gene might down regulate the expression ofBcl-2.

Keywords

telomere telomerase Bcl-2

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References

  1. 1.
    Greider, C. W., Telomere length regulation, Annu. Rev. Biochemistry, 1996, 65: 337.CrossRefGoogle Scholar
  2. 2.
    Autexier, C., Greider, C. W., Mutational analysis of tetrahymena telomerase RNA: identification of residues affection telomerase activityin vitro, Nuckeic Acids Res., 1998, 26: 787.CrossRefGoogle Scholar
  3. 3.
    Shay, J. W., Bacchetti, S., Asurvey of telomerase activity in human cancer, Eur. J. Cancer, 1997, 33: 787.CrossRefGoogle Scholar
  4. 4.
    Rusconi, J. C., Hays, R., Cogan, R. L., Programmed cell death and pathway in Drosphila, Cell Death Differ, 2000, 7: 1063.CrossRefGoogle Scholar
  5. 5.
    Savill, J., Fadok, V., Corpse clearance defines the meaning of cell death, Nature, 2000, 407: 784.CrossRefGoogle Scholar
  6. 6.
    Kam, P. C. A., Ferch, N. I., Apoptosis: mechanism and clinical implications, Anaesthesia, 2000, 55: 1081.CrossRefGoogle Scholar
  7. 7.
    Chen, M. C., Hsu, T. L., Luh, T. Y. et al., Overexpression ofBcl-2 enhances LIGHTand interferongamma-mediated apoptosis in Hep3BT2 cells, J. Biol. Chem., 2000, 275: 38794.CrossRefGoogle Scholar
  8. 8.
    Mandai, M., Kumar, R.,Bcl-2 expression regulates sodium butyrate-induced apoptosis in human MCF-7 breast cancer cells, Cell Growth Diff., 1996, 7: 311.Google Scholar
  9. 9.
    Mandai, M., Maggirwar, S. B., Sharma, N. et al.,Bcl-2 prevents CD95 (Fas/ApoH l)-induced degradation of lamin B and poly (ADP-ribose) polymerase and restores the NF-kappa B signaling pathway, J. Biol. Chem., 1996, 271: 30354.CrossRefGoogle Scholar
  10. 10.
    Mandai, M., Kumar, R.,Bcl-2 modulates telomerase activity, J. Biol. Chem., 1997, 272: 14183.CrossRefGoogle Scholar
  11. 11.
    Fu, W., Begley, J. G., Killen, M. W. et al., Anti-apoptotic role of telomerase on pheochromocytoma cells, J. Biol. Chem., 1999, 274: 7264.CrossRefGoogle Scholar
  12. 12.
    Ren, J. G., Xia, H. L., Dai, Y. R., The changes in telomerase activity and telomere length in HeLa cells undergoing apoptosis induced by sodium butyrate, Chinese Science Bulletin, 2000, 45: 1861.Google Scholar
  13. 13.
    Hildeman, D. A., Mitchell, T., Teague, T. K. et al., Reactive oxygen species regulate activation-induced T-cell apoptosis, Immunity, 1999, 10: 735.CrossRefGoogle Scholar
  14. 14.
    Hultdin, M., Gronlund, E., Norrback, K. F. et al., Telomere analysis by fluorescencein situ hybridization and flow-cytometry, Nucleic Acids Res., 1998, 26: 3651.CrossRefGoogle Scholar
  15. 15.
    Mandai, M., Kumar, R.,Bcl-2 expression regulates sodium butyrate-induced apoptosis in human MCF-7 breast-cancer cells, Cell Growth Diff., 1996, 7: 311.Google Scholar
  16. 16.
    Ohmura, Y., Aoe, M., Hndou, A. et al., Telomerase activity andBcl-2 expression in non-small cell lung cancer, Clinical Cancer Res., 2000, 6: 2980.Google Scholar
  17. 17.
    Johnson, V. L., Cooper, I. R., Jenkins, J. R. et al., Effects of differential overexpression ofBcl-2 on apoptosis, proliferation, and telomerase activity in Jurkat T cells, Exp. Cell Res., 1999, 251: 175.CrossRefGoogle Scholar
  18. 18.
    Ren, J. G., Xia, H. L., Just, T. et al., Hydroxyl radical-induced apoptosis in human tumor cells is associated with telomere shortening but not telomerase inhibition and caspase activation, FEBS Letters, 2001, 488: 123.CrossRefGoogle Scholar
  19. 19.
    Ren, J. G., Xia, H. L., Tian, Y. M. et al., Expression of telomerase inhibits hydroxyl radical-induced apoptosis in normal telomerase negative human lung fibroblasts, FEBS Letters, 2001, 488: 133.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2001

Authors and Affiliations

  • Jing Chen
    • 1
    • 2
  • Jianguo Ren
    • 1
  • Jianyu Zheng
    • 2
  • Guoping Cai
    • 1
  • Yaoren Dai
    • 1
  1. 1.Department of Biological Sciences and BiotechnologyTsinghua University, National Key Laboratory of Biomembrance and Membrance BiotechnologyBeijingChina
  2. 2.Institute for Molecular BiologyNankai UniversityTianjinChina

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