Abstract
TheGbKTN1 gene was isolated from 10 DPA fiber cells ofGossypium barbadense using 5′RACE/3′RACE. Full-length cDNA of this gene is 2006 bp, including a 113 bp of 5′untranslated region, a 1563 bp of an open reading frame (ORF), and a 327 bp of 3′untranslated region (excluding the stop codon TAA). The ORF ofGbKTN1 encodes a 521-amino acid protein with a predicted size of 55 kD. Near C-terminal of the deduced protein there is a putative ATP binding site between amino acid residues from 233 to 414. Southern blot analysis indicated that theGbKTN1 was a single copy gene inG. barbadense. Combining semi-quantitative RT-PCR with Southern blot hybridization revealed thatGbKTN1 expressed in all the organs detected such as roots, stems, leaves and fibers. However, the mRNA ofGbKTN1 was the most abundant in fiber cells, while it was the lowest in leaves. TheGbKTN1 cDNA was transformed intoS. pombe to verify its function on cell elongation. Results showed that most yeast cells over expressingGbKTN1 gene were elongated dramatically with an average length increase of 2.18 times than that of the non-induced cells. Even the morphology of some yeast cells appeared irregularly. To the best of our knowledge this is the first evidence that KTN1 is correlated with cell elongationin vivo.
Similar content being viewed by others
References
Graves, D. A., Steward, J. M., Chronology of the differentiation of cotton (Gossypium hirsutum L.) fiber cells, Planta, 1988, 175: 54–258.
Arioli, T., Peng, L., Betzner, A. S. et al., Molecular analysis of cellulose biosynthesis inArabidopsis, Science, 1998, 279: 717–720.
Burk, D. H., Liu, B., Zhong, R. et al., A katanin-like protein regulates normal cell wall biosynthesis and cell elongation, Plant Cell, 2001, 13: 807–827.
Burk, D. H., Ye, Z. H., Alteration of oriented deposition of cellulose microfibrils by mutation of a katanin-like microtubule-severing protein, Plant Cell, 2002, 14: 2145–2160.
Ha, M. A., MacKinnon, I. M., Sturcova, A. et al., Structure of cellulose-deficient secondary cell walls from the irx3 mutant ofArabidopsis thaliana, Phytochemistry, 2002, 61: 7–14.
Nicol, F., His, I., Jauneau, A. et al., A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation inArabidopsis, EMBO J., 1998, 17: 5563–5576.
Zuo, J., Niu, Q. W., Nishizawa, N. et al., KORRIGAN, anArabidopsis endo-1, 4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis, Plant Cell, 2000, 12: 1137–1152.
Kost, B., Mathur, J., Chua, N. H., Cytoskeleton in plant development, Curr. Opin. Plant Biol., 1999, 2: 462–470.
Kropf, D. L., Bisgrove, S. R., Hable, W. E., Cytoskeletal control of polar growth in plant cells, Curr. Opin. Cell Biol., 1998, 10: 117–122.
Quader, H., Herth, W., Ryser, U. et al., Cytoskeletal elements in cotton seed hair developmentin vitro: Their possible regulatory role in cell wall organization, Protoplasma, 1987, 137: 56–62.
Seagull, R., The effects of microtubules and microfilament disrupting agents on cyoskeletal arrays and wall deposition in developing cotton fibers, Protoplasma, 1990, 159: 44–59.
Baskin, T. L., The cyoskeleton, Biochemistry and Molecular Biology of Plants. (eds. Buchanan, B. B., Gruissem, W., Jones, R. L.) Rockville, M.D: American Society of Plant Physiologists, 2000, 202–258
Bichet, A., Desnos, T., Turner, S. et al., BOTERO1 is required for normal orientation of cortical microtubules and anisotropic cell expansion inArabidopsis, Plant J. 2001, 25: 137–148.
Vale, R. D., Severing of stable microtubules by a mitotically activated protein inXenopus egg extracts, Cell, 1991, 64: 827–839.
McNally, F. J., Okawa, K., Iwamatsu, A. et al., Katanin, the microtubule-severing ATPase, is concentrated at centrosomes, J. Cell. Sci., 1996, 109: 561–567.
McNally, F. J., Thomas, S., Katanin is responsible for the M-phase microtubule-severing activity inXenopus eggs, Mol. Biol. Cell, 1998, 9: 1847–1861.
Ahmad, F. J., Yu, W., McNally, F. J. et al., An essential role for katanin in severing microtubules in the neuron, J. Cell. Biol., 1999, 145: 305–315.
McNally, K. P., Buster, D., McNally, F. J., Katanin-mediated microtubule severing can be regulated by multiple mechanisms, Cell Motility & Cytoskeleton, 2002, 53: 337–349.
Stoppin-Mellet, V., Gaillard, J., Vantard, M., Plant katanin, a microtubule severing protein, Cell Biol. Int., 2003, 27: 279.
Cotton Research Institute, Chinese Academy of Agricultural Sciences, Cotton Cultivation in China (in Chinese), 1st ed., Shanghai: Shanghai Science & Technology Press, 1983, 123–133.
Burton, R. A., Gibeaut, D. M., Bacic, A. et al., Virus-induced silencing of a plant cellulose synthase gene, Plant Cell, 2000, 12: 691–706.
Maundrell, K., Thiamine-repressible expression vectors pREP and pRIP for fission yeast, Gene, 1993, 123: 127–130.
Confalonieri, F., Duguet, M., A 200-amino acid ATPase module in search of a basic function, Bioessays, 1995, 17: 639–650.
Xia, G., Ramachandran, S., Hong, Y. et al., Identification of plant cytoskeleton, cell cycle-related and polarity-related genes usingSchizosaccaromycespombe, Plant J., 1996, 10: 761–769.
Hartman, J. J., Mahr, J., McNally, K. et al., Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit, Cell, 1998, 93: 277–287.
Srayko, M., Buster, D. W., Bazirgan, O. A. et al., MEI-1/MEI-2 katanin-like microtubule severing activity is required forCaenorhabditis elegans meiosis, Genes Dev., 2000, 14: 1072–1084.
Carpita, N., McCann, M., The cell wall, Biochemistry and Molecular Biology of Plants (eds. Buchanan, B. B., Gruissem, W., Jones, R. L.), Rockville, M. D: American Society of Plant Physiologists, 2000, 52–108.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Li, W., Wang, Z. & Jia, S. Effect of GbKTN1 fromGossypium barbadense on cell elongation of fission yeast (Schizosaccharomyces pombe). Chin. Sci. Bull. 49, 23–28 (2004). https://doi.org/10.1007/BF02901738
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02901738