Estimation of crude fiber in dehulled soybeans
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The determination of crude fiber in soybeans is a lengthy and time-consuming procedure which requires extraction, digestion, and incineration and is not suitable as a routine processing-control tool. A short control procedure has been developed, employing photomicroscopy, which is based on the characteristics of soybean hull cellulose to rotate plane-polarized light. The sample is mounted on a microscope slide, treated with trichloro-acetic acid, and placed in a polarizing projection microscope; the image is compared with a series of standard photomicrographs. The amount of hulls present in the sample is measured quantitatively, and an estimation is made on the crude fiber. The crude fiber estimation on de-hulled soybeans can be made in 15 min compared with 8 to 12 hr by using the official crude fiber method.