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Chinese Science Bulletin

, Volume 46, Issue 24, pp 2086–2088 | Cite as

Electrophysiology and behavior feedback of diamondback moth,Plutella xylostella, to volatile secondary metabolites emitted by Chinese cabbage

  • Han Baoyu
  • Zhang Zhongning
  • Fang Yuling
Notes

Abstract

The volatiles, absorbed by Porapak Q and eluted by hexane, from Chinese cabbage,Brassica campestris chinesis (L.) Makino. var.communis Tsen et Lee, have been identified as allyl isothiocyanate, Z-3-hexenyl acetate, 3,7-dimethyl-l,3,6-octatriene, 2,5-hexanediol, Z-3-hexen-1-ol, nonanol, β-myrcene, α-pinene, E-2-hexen-1-ol, D-limonene, Z-3-hexenyl propanoate, linalool, geraniol, E-4-hexen-1-ol, Z-3-hexenyl isovalerate, α-terpinene, β-caryophyllene, 3-carene and α-caryophyllene, by their mass spectra and retention times in comparison with authentic samples. The first five chemicals are the main components. All components can elicite electroantennogram (EAG) responses of dia-mondback moth (DBM),Plutella xylostella, in which allyl isothiocyanate and C6alcohols and esters, i.e. Z-3-hexen-l-ol, E-2-hexen-1-ol, E-4-hexen-1-ol, Z-3-hexenyl isovalerate and 2,5-hexanediol elicite stronger EAG responses than other components, and EAG responses of female moths to them are slightly larger than those of male moths. However, EAG responses elicited by terpenes are smaller, and EAG responses from females to terpenes are smaller than those from males, but β-myrcene can evoke stronger EAG responses from both females and males. Volatiles from intact Chinese cabbage, allyl isothiocyanate, 2,5-hexanediol and Z-3-hexenyl isovalerate intensely cause DBM directional flying and landing in wind tunnel, in which volatiles from intact Chinese cabbage have the strongest activity, and the next is allyl isothiocyanate. Allyl isothiocyanate is more attractive to females than to males a bit. As for α-terpinene and other subordinate components, EAG and wind tunnel test make sure they have weak activity.

Keywords

Chinese cabbage diamondback moth volatiles elec-troantennogram wind tunnel directional fly 

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References

  1. 1.
    Reddy, G. V. P., Guerrero, A., Pheromone-based integrated pest management to control the diamondback mothPlutella xylostella in cabbage fields, Pest Manag. Sci., 2000, 56: 882.CrossRefGoogle Scholar
  2. 2.
    Liu, S. S., Wang, X. G., Shi, Z. H. et al., Biology ofOomyzus sokolowskii and effect of temperature on its population parameters, Acta Entomologica Sinica (in Chinese), 2000, 43 (2): 159.Google Scholar
  3. 3.
    Agelopoulos, N. A., Keller, M. A., Plant-natural enemy association in the tritrophic systemCotesia rubecuba-Pieris rapae-Brassicaceae (Cruciferae). III. Collection and identification of plant and frass volatiles, J. Chem. Ecol., 1994c, 20 (8): 1955.CrossRefGoogle Scholar
  4. 4.
    Blaakmeer, A., Geervliet, J. B. F., van Loon, J. J. A. et al., Comparative headspace analysis of cabbage plants damaged by two species ofPieris caterpillars:consequences for in-flight host location byCotesia parasitoids, Entomol. Exp. Appl., 1994, 73: 175.CrossRefGoogle Scholar
  5. 5.
    Geervliet, J. B. F., Posthumus, M. A., Vet, L. E. M. et al., Comparative analysis of headspace volatiles from different caterpillar-infested or uninfested food plants ofPieris species, J. Chem. Ecol., 1997, 23 (12): 2935.CrossRefGoogle Scholar
  6. 6.
    Dong, W. X., Wang, R.,Zhang, Z. N., Electroantennal responses of parasitoid (Microplitis mediator) to cotton plant volatiles, Acta Entomologica Sinica (in Chinese), 2000, 43(Suppl.): 119.Google Scholar
  7. 7.
    Takken, W., Dekker, T., Wijnholds, Y. G., Odor-mediated flight behaviorof Anopheles gambiae GilesSensu Stricto and An. stephensi Liston in response to CO2, Acetone, and 1-Octen-3-ol (Diptera: Culicidae), J. Insect Behav., 1997, 10(3): 395.CrossRefGoogle Scholar
  8. 8.
    Du, Y. J., Poppy, G. M., Powell, W., Relative importance of semiochemicals from first and second trophic levels in host foraging behavior ofAphidius ervi, J. Chem. Ecol., 1996, 22(9): 1591CrossRefGoogle Scholar
  9. 9.
    Du, Y. J., Poppy, G. M., Powell, W. et al., Identification of semio-chemicals released during aphid deeding that attract parasitoidAphidius ervi, J. Chem. Ecol., 1998, 24(8): 1355.CrossRefGoogle Scholar
  10. 10.
    Turlings, T. C. J., Bernasconi, M., Bertossa, R. et al., The induction of volatiles in maize by three herbivore species with different feeding habits: possible consequences for their natural enemies, Biol. Control, 1998, 11: 122.CrossRefGoogle Scholar
  11. 11.
    Raguso, R. A., Light, D. M., Electroantennogram responses of maleSphinx perelegans hawkmoths to floral and “greenleaf volatiles”, Entomol. Exp. Appl., 1998, 86: 287.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2001

Authors and Affiliations

  1. 1.Institute of ZoologyChinese Academy of SciencesBeijingChina
  2. 2.Key Laboratory of Tea Biotechnology of Ministry of Agriculture of Anhui Agricultural UniversityHefeiChina

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