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Chinese Science Bulletin

, 46:1638 | Cite as

In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)

  • Qiya Zhang
  • Zhenqiu Li
  • Jianfang Gui
Notes

Abstract

Forty embryonic hearts were taken out by anatomical needle from denuded embryos of the ovoviviparity guppy fish that were dechorioned by mechanic method or by trypsin digestion, and werein vitro cultured. In the cultured hearts, 80% have maintained beatingin vitro for 4 weeks, and the longest record for beating was 142 d. Owing to fish embryo transparency, beating frequency and blood color changes are easily viewed from the embryonic hearts under a dissecting microscope. The current study established thein vitro culture method of embryonic hearts in guppy fish, which can be used as a model for the study of heart and cardiovascularf system in vertebrates.

Keywords

guppy fish embryo heart in vitro culture 

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Copyright information

© Science in China Press 2001

Authors and Affiliations

  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiololgyChinese Academy of SciencesWuhanChina

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