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Chinese Science Bulletin

, Volume 46, Issue 7, pp 582–585 | Cite as

High expression of human serum albumin in milk of transgenic mice directed by the goat β-casein gene promoter region

  • Ying Huang
  • Ying Huang
  • Zan Huang
  • Jingbin Yan
  • Zhanlu Ma
  • Min Sheng
  • Zhaorui Ren
  • Yitao Zeng
  • Shuzhen Huang
Notes

Abstract

We have constructed a mammary gland expression vector that contained the goat β-casein gene promoter, 5′upstream regulatory region, exons 1,2, intron 1 as well as the human serum albumin (hALB) mini-gene (including the full-long sequences of hALB cDNA and its intron 1). Injection of the vector into mouse tail veins showed that the recombinant construct was expressed only in mammary glands. The vector was microinjected into the mouse fertilized eggs, followed by transferring the eggs into the foster mice. 33 F0 mice were obtained. Of the 33, 8 mice (5♀, 3 ♂) were transgenic with hALB gene integration identified by PCR as well as Southern blot hybridization. The integration rate was 24.2% (8/33). Western blot analysis showed that 3 female transgenic mice had hALB expression in their milk. The hALB contents in milk reached 3.54, 0.21 and 3.03 g/L, respectively.

Keyworkds

human serum albumin goat β-casein transgenic mice mammary gland 

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Copyright information

© Science in China Press 2001

Authors and Affiliations

  • Ying Huang
    • 1
  • Ying Huang
    • 1
  • Zan Huang
    • 1
  • Jingbin Yan
    • 1
  • Zhanlu Ma
    • 1
  • Min Sheng
    • 1
  • Zhaorui Ren
    • 1
  • Yitao Zeng
    • 1
  • Shuzhen Huang
    • 1
  1. 1.Shanghai Institute of Medical GeneticsShanghai Children’s HospitalShanghaiChina

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