Interaction of 2-deoxy-d-glucose and adenine with phosphate anion uptake in yeast
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The transport of inorganic phosphate anions into yeast cells (after preincubation with glucose; fructose or another metabolizable sugar, and in the presence of glucose) shows two kinetic components with half-saturation constants of 40 μmol/L and 2.4 mmol/L. The uptake was strikingly stimulated by 2-deoxy-d-glucose (2-dGle) at lower concentrations but inhibited above, 100 mmol/L. A similar stimulation was caused by adenine (0.01–1 mmol/L) and a very small one by uracil and inorganic sulfate. It is suggested that either a phosphorylation reaction accompanies the transport (2-dGlc) or that some compounds stimulate the H+-ATPase more than inorganic phosphate itself and thus increase its rate of transport.
KeywordsAdenine Yeast Cell Trehalose Phosphate Transport Phosphate Anion
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