Folia Microbiologica

, 37:401 | Cite as

Interaction of 2-deoxy-d-glucose and adenine with phosphate anion uptake in yeast

  • A. Kotyk


The transport of inorganic phosphate anions into yeast cells (after preincubation with glucose; fructose or another metabolizable sugar, and in the presence of glucose) shows two kinetic components with half-saturation constants of 40 μmol/L and 2.4 mmol/L. The uptake was strikingly stimulated by 2-deoxy-d-glucose (2-dGle) at lower concentrations but inhibited above, 100 mmol/L. A similar stimulation was caused by adenine (0.01–1 mmol/L) and a very small one by uracil and inorganic sulfate. It is suggested that either a phosphorylation reaction accompanies the transport (2-dGlc) or that some compounds stimulate the H+-ATPase more than inorganic phosphate itself and thus increase its rate of transport.


Adenine Yeast Cell Trehalose Phosphate Transport Phosphate Anion 
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  1. Alonso A., Kotyk A.: Apparent half-lives of sugar transport proteins inSaccharomyces cerevisiae.Folia Microbiol. 23, 118–125 (1978).Google Scholar
  2. Benito B., Moreno E., Lagunas R.: Half-life of the plasma membrane ATPase and its activating system in resting yeast cells.Biochim. Biophys. Acta 1063, 265–268 (1991).PubMedCrossRefGoogle Scholar
  3. Borst-Pauwels G.F.W.H., Loef H.W., Havinga E.: The uptake of radioactive phosphate by yeast. II. The primary phosphorylation products.Biochim. Biophys. Acta 65, 407–411 (1962).PubMedCrossRefGoogle Scholar
  4. Van Den Broek P.J.A., Van Steveninck J.: Kinetic analysis of 2-deoxy-d-glucose uptake inSaccharomyces fragilis.Biochim. Biophys. Acta 649, 395–309 (1983).Google Scholar
  5. Cockburn M., Earnshaw P., Eddy A.A.: The stoichiometry of the absorption of protons with phosphate andl-glutamate by yeast of the genusSaccharomyces cerevisiae.Biochem. J. 146, 705–712 (1975).PubMedGoogle Scholar
  6. Franzusoff A., Cirillo V.P.: Uptake and phosphorylation of 2-deoxy-d-glucose by wild-type and single-kinase strains ofSaccharomyces cerevisiae.Biochim. Biophys. Acta 688, 295–304 (1982).PubMedCrossRefGoogle Scholar
  7. Horak J., Kotyk A., Rihova L.: Stimulation of amino acid transport inSaccharomyces cerevisice by metabolic inhibitors.Folia Microbiol. 23, 286–291 (1978).CrossRefGoogle Scholar
  8. Jaspers H.T.A., van Steveninck J.: Active transport ofl-sorbose and 2-deoxy-d-galactose inSaccharomyces fragilis.Biochim. Biophys. Acta 469, 292–300 (1977).PubMedCrossRefGoogle Scholar
  9. Knotkova A., Kotyk A.: Role of sugars in phosphate transport in baker’s yeast.Folia Microbiol. 17, 251–260 (1972).CrossRefGoogle Scholar
  10. Knotkova A., Kotyk A.: Dependence of phosphate transport in yeast on glycolytic substrates.Folia Microbiol. 26, 377–381 (1981).CrossRefGoogle Scholar
  11. Kotyk A.: Effects of yeast suspension density on the accumulation ratio of transported solutes.Yeast 3, 263–270 (1987).PubMedCrossRefGoogle Scholar
  12. Kotyk A., Dvorakova M.: Are proton symports in yeast directly linked to H+-ATPase acidification?Biochim. Biophys. Acta 1104, 293–298 (1992).PubMedCrossRefGoogle Scholar
  13. Kotyk A., Michalianicova D.: Uptake of trehalose bySaccharomyces cerevisiae.J. Gen. Microbiol. 110, 323–332 (1979).PubMedGoogle Scholar
  14. Kotyk A., Horak J., Knotkova A.: Transport protein synthesis in nongrowing yeast cells.Biochim. Biophys. Acta 698, 243–251, (1982).PubMedGoogle Scholar
  15. Poncova M., Kotyk A.: Interaction of monosaccharides with amino acid uptake bySaccharomyces cerevisiae:Curr. Mod. Biol. 1, 189–191 (1967).PubMedGoogle Scholar
  16. Roomans G.M., Blasco F., Borst-Pauwels G.W.F.H.: Cotransport of phosphate and sodium by yeast.Biochim. Biophys. Acta 467, 65–71 (1977).PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1992

Authors and Affiliations

  • A. Kotyk
    • 1
  1. 1.Department of Membrane Transport, Institute of PhysiologyCzechoslovak Academy of SciencesPrague 4

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