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Virchows Archiv B

, Volume 10, Issue 1, pp 145–151 | Cite as

Autoradiographic investigation on granulation tissue with radioactive and nonradioactive thymidine

  • B. Helpap
  • H. Cremer
Article
  • 11 Downloads

Summary

Prelabeling studies with H-3-thymidine in granulation tissue demonstrated, that fibroblasts are predominantly of hematogenic origin. However, the interpretation of those prelabeling results is difficult, because, proliferating fibroblasts can reutilize labeled DNA-fragments from damaged cells.

To exclude DNA-reutilization by proliferating fibroblasts, prelabeled mice with granulation tissue in the liver, received injections of nonradioactive thymidine 1 hour after the application of H-3-thymidine till the end of the investigation time.

In comparison with results after prelabeling mice without nonradioactive thymidine, there was no difference in the labeling index of leucocytes, histiocytes and fibroblasts in the granulation tissue. Only the number of the silver grains in all three celltypes decreased in the same fashion after injection of nonradioactive thymidine.

Indeed, the results permit the conclusion, that wound-fibroblasts as well as histiocytes develop from immigrated and transformated mononuclear precursor cells. The latter cells have reutilized low molecular DNA-fragments in their hematogenic origin. They are labeled as well by initial injection of thymidine as by reutilization of DNA-fragments in their hematogenic origin.

Keywords

Granulation Tissue Tritiated Thymidine Autoradiographic Investigation Hematogenic Origin Mononuclear Precursor Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Autoradiographische Untersuchungen am Granulationsgewebe mit radioaktiv markiertem und unmarkiertem Thymidin

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • B. Helpap
    • 1
  • H. Cremer
    • 1
  1. 1.Pathologisches Institut der Universität BonnDeutschland

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