Summary
Prelabeling studies with H-3-thymidine in granulation tissue demonstrated, that fibroblasts are predominantly of hematogenic origin. However, the interpretation of those prelabeling results is difficult, because, proliferating fibroblasts can reutilize labeled DNA-fragments from damaged cells.
To exclude DNA-reutilization by proliferating fibroblasts, prelabeled mice with granulation tissue in the liver, received injections of nonradioactive thymidine 1 hour after the application of H-3-thymidine till the end of the investigation time.
In comparison with results after prelabeling mice without nonradioactive thymidine, there was no difference in the labeling index of leucocytes, histiocytes and fibroblasts in the granulation tissue. Only the number of the silver grains in all three celltypes decreased in the same fashion after injection of nonradioactive thymidine.
Indeed, the results permit the conclusion, that wound-fibroblasts as well as histiocytes develop from immigrated and transformated mononuclear precursor cells. The latter cells have reutilized low molecular DNA-fragments in their hematogenic origin. They are labeled as well by initial injection of thymidine as by reutilization of DNA-fragments in their hematogenic origin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Allgöwer, M.: The cellular basis of wound repair. Springfield (Ill.) : Charles C. Thomas 1956.
Bryant, B. J.: Reutilization of leukocyte DNA by cells of regenerating liver. Exp. Cell Res.27, 70–79 (1962).
— In vivo reutilization of the DNA thymidine of necrotized liver cells by cells of testis and intestine. Exp. Cell Res.32, 209–212 (1963).
— Reutilization of lymphocyte DNA by cells of intestinal crypts and regenerating liver. J. Cell Biol.18, 515–523 (1963).
Büchner, Th.: Entzündungszellen im Blut und im Gewebe. Veröffentlichungen aus der morphologischen Pathologie, H. 86. Stuttgart: Gustav Fischer 1971.
— Junge-Hülsing, G., Wagner, H., Oberwittler, W., Hauss, W. H.: Zur Herkunft und Entstehung von Entzündungszellen im Granulationsgewebe. Autoradiographische Untersuchungen mit H-3-Thymidin am Wattegranulom der Ratte. Klin. Wschr.48, 867–872 (1970).
— Müller, U. St., Hauss, W. H.: Zur haematogenen Herkunft von Zellen des Granulationsgewebes (Zellkinetische autoradiographische Studie am Fremdkörpergranulom und der Wundheilung bei der Ratte). Verh. dtsch. Ges. inn. Med.76, 514–517 (1970).
— Zur prospektiven Potenz mononukleärer Blutzellen bei der granulierenden Entzündung. Verh. dtsch. Ges. Path.54, 416–421 (1970).
Cottier, H., Odartchenko, N., Feinendegen, L. E., Keiser, G., Bond, V. P.: Autoradiographische Untersuchungen über die Entkernung der Erythroblasten nach in vivo- Markierung mit H-3-Thymidin. Schweiz. med. Wschr.93, 1061–1075 (1963).
Diderholm, H., Fichtelius, K. E., Linder, O.: Availability time of H-3-label after administration of H-3-thymidine in vivo. Exp. Cell Res.27, 431–435 (1962).
Dumont, A. E.: Fibroplasia: A Sequel to lymphocyte exudation in the inflammatory process, p. 535–558. New York and London: Academic Press 1965.
Feinendegen, L. E.: Tritium labeled molecules in biology and medicine, p. 267–269. New York and London: Academic Press 1967.
— Bond, V. P., Hughes, W. L.: 125-J-DU (5-iodo-2′-deoxyuridine) in autoradiographic studies of cell proliferation. Exp. Cell Res.43, 107–119 (1966).
Fichtelius, K. E., Diderholm, H.: Autoradiographic analysis of the accumulation of lymphocytes in wounds. Acta path. microbiol. scand.52, 11–18 (1961).
— Groth, O.: Reutilization of deoxyribonucleic acid from cells other than leucocytes. Nature (Lond.)200, 587–588 (1963).
Gerecke, D.: Persönliche Mitteilung.
Helpap, B., Cremer, H.: Proliferationsvorgänge in der traumatisch-geschädigten Leber. Virchows Arch. Aqt. B Zellpath.6, 365–366 (1970).
— Zellkinetische Untersuchungen zur Wundheilung der Mäuseleber. Virchows Arch. Abt. B Zellpath.10, 134–144 (1972).
— Grouls, V.: Reutilisation von markierten DNS-Bausteinen bei der Wundheilung. Naturwissenschaften58, 574–575 (1971).
Hulliger, L.: Über die unterschiedliche Entwicklungsfähigkeit des Blutes und der Lymphe in vitro. Virchows Arch. path. Anat.329, 289–318 (1956).
Leder, L. D.: Der Blutmonocyt. Berlin-Heidelberg-New York: Springer 1967.
Maruyama, Y.: Reutilization of thymidine during death of a cell. Nature (Lond.)201, 93–94 (1964).
Robinson, S. H., Brecher, G.: Delayed incorporation of tritiated thymidine into DNA. Science142, 392–393 (1963).
— Lourie, I. S., Haley, J. E.: Leukocyte labeling in rats during and after continuous infusion of tritiated thymidine: implications for lymphocyte longevity and DNA-reutilization. Blood26, 281–295 (1965).
Roos, B.: Makrophagen: Herkunft, Entwicklung und Funktion. In: Handbuch der allgemeinen Pathologie, Bd. 7, Teil 3. Berlin-Heidelberg-New York: Springer 1970.
Schultze, B.: Die Orthologie und Pathologie des Nukleinsäure- und Eiweißstoffwechsels der Zelle im Autoradiogramm. In: Handbuch der allgemeinen Pathologie, Bd. 2, Teil 5. Berlin-Heidelberg-New York: Springer 1968.
Author information
Authors and Affiliations
Additional information
Herrn Prof. Dr. W. Maurer zum 65. Geburtstag gewidmet.
Mit Unterstützung der Deutschen Forschungsgemeinschaft.
Rights and permissions
About this article
Cite this article
Helpap, B., Cremer, H. Autoradiographische Untersuchungen am Granulationsgewebe mit radioaktiv markiertem und unmarkiertem Thymidin. Virchows Arch. Abt. B Zellpath. 10, 145–151 (1972). https://doi.org/10.1007/BF02899724
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02899724