Advertisement

Virchows Archiv B

, Volume 62, Issue 1, pp 25–34 | Cite as

Epithelial differentiation in gliomas, meningiomas and choroid plexus papillomas

  • F. F. Cruz-Sanchez
  • M. Garcia-Bachs
  • M. L. Rossi
  • S. Rodriguez-Prados
  • I. Ferrer
  • H. B. Coakham
  • J. C. Ferreres
  • J. Figols
  • A. Palacin
Original Articles

Summary

The immunohistological findings using antibodies to different intermediate filaments (glial fibrillary acidic protein, vimentin and two types of cytokeratin) and epithelial membrane antigen are described in 89 gliomas, 19 meningiomas and 8 choroid plexus papillomas (CPPs) from adult patients. All the patients had total or subtotal surgical excision of their tumours with clinical follow up for between 3 and 7 years. The immunohistological results were correlated with the histological features and patient survival. Tumours other than low grade astrocytomas, oligodendrogliomas and anaplastic ependymomas expressed one or more epithelial markers. This immunohistological evidence of epithelial differentiation in the absence of histological epithelial features in gliomas confirms that the two are not necessarily correlated. It is concluded that the expression of epithelial markers in some intradural tumours may reflect aberrant differentiation related to the degree of anaplasia in poorly differentiated astrocytomas and glioblastomas. All the patients with anaplastic epithelial marker-positive gliomas died within 1 year, whereas only 68% of patients with marker-negative tumours died within the follow-up period. In ependymomas and meningiomas, the expression of epithelial markers may reflect their histogenesis, while in malignant CPPs such expression could denote either their aberrant differentiation or histogenetic derivation.

Key words

Brain tumour Cytokeratin Epithelial membrane antigen Glial fibrillary acidic protein 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alvarez F, Roda MJ, Romero MP, Morales C, Sarmiento MA, Blazquez MG (1987) Malignant and atypical meningiomas: a reappraisal of clinical, histological and computed tomographic features. Neurosurgery 20:688–694PubMedCrossRefGoogle Scholar
  2. Bignami A, Raju T, Dahl D (1982) Localization of vimentin, the non specific intermediate filament protein, in embryonal glia and in early differentiating neurons. In vivo and in vitro immunofluorescence study of the rat embryo with vimentin and neurofilament antisera. Dev Biol 91:286–295PubMedCrossRefGoogle Scholar
  3. Choi BH, Kim RC (1984) Expression of glial fibrillary acidic protein in immature oligodendroglia. Science 223:407–409PubMedCrossRefGoogle Scholar
  4. Churg A (1985) Immunohistochemical staining for vimentin and keratin in malignant mesothelioma. Am J Surg Pathol 9:360–365PubMedCrossRefGoogle Scholar
  5. Coakham HB, Garson JA, Allan PM (1985) Immunohistological diagnosis of central nervous system tumours using a monoclonal antibody panel. J Clin Pathol 38:165–173PubMedCrossRefGoogle Scholar
  6. Coffin CM, Wick MR, Braun JT, Dehner LP (1986) Choroid plexus neoplasm. Clinico-pathologic and immunohistochemical studies. Am J Surg Pathol 10:394–404PubMedCrossRefGoogle Scholar
  7. Cosgrove MM, Rich KA, Martin SE (1988) Keratin intermediate filament expression in neoplastic and non neoplastic astrocytes. Lab Invest 60:20AGoogle Scholar
  8. Cosgrove M, Fitzgibbons PL, Sherrod A, Chandrasoma PT, Martin SE (1989) Intermediate filament expression in astrocyte neoplasms. Am J Surg Pathol 13:141–145PubMedCrossRefGoogle Scholar
  9. Cruz-Sanchez FF, Rossi ML, Esiri MM, Reading M (1988a) Epithelial membrane antigen expression in ependymomas. Neuropathol Appl Neurobiol 14:197–205PubMedGoogle Scholar
  10. Cruz-Sanchez FF, Rossi ML, Hughes JT (1988b) An immunohistological study of the cytogenesis and differentiation of ependymomas. Neuropathol Appl Neurobiol 14:250–251Google Scholar
  11. Cruz-Sanchez FF, Rossi ML, Hughes JT, Cervos-Navarro J (1988c) An immunohistological study of 66 ependymomas. Histopathology 13:443–454PubMedCrossRefGoogle Scholar
  12. Cruz-Sanchez FF, Rossi ML, Hughes JT, Coakham HB, Figols J, Eynaud PM (1989) Choroid plexus papillomas: an immunohistological study of 16 cases. Histopathology 15:61–69PubMedGoogle Scholar
  13. Doglioni C, Dellorto P, Coggi G, Yuzzolino P, Bontempini L, Viale G (1987) Choroid plexus tumours. Immunocytochemical study with particular reference to the co-expression of intermediate filament proteins. Am J Pathol 127:519–529PubMedGoogle Scholar
  14. Duffy PE, Graf L, Huang Y-Y, Rapport MM (1979) Glial fibrillary acidic protein in ependymomas and other brain tumours. J Neurol Sci 401:33–146Google Scholar
  15. Duffy PE, Huang Y-Y, Rapport MM, Graf L (1980) Glial fibrillary acidic protein in giant cell tumours and brains and other gliomas. A possible relationship to malignancy, differentiation and pleomorphism of glia. Acta Neuropathol (Berl) 52:51–57CrossRefGoogle Scholar
  16. Ellis IO, Robbins RA, Elston CW, Blarney RW, Ferry B, Dalwin RW (1985) A monoclonal, antibody NRC-11 antibody raised to breast carcinoma. I. Production and immunohistological characterization. Histopathology 9:729–739CrossRefGoogle Scholar
  17. Furness PN, Lowe J, Tarrant GS (1990) Subepithelial basement membrane deposition and intermediate filament expression in choroid neoplasm and ependymomas. Histopathology 16:251–255PubMedCrossRefGoogle Scholar
  18. Herpers MJHM, Budka H (1984) Glial fibrillary acidic protein (GFAP) in oligodendroglioma and transitional oligoastrocytoma as subtypes of oligodendroglioma. Acta Neuropathol (Berl) 64:265–272CrossRefGoogle Scholar
  19. Herpers MJHM, Ramaekers FCS, Aldeweireldt J, Moesker O, Sloff J (1986) Co-expression of glial fibrillary acidic protein- and vimentin intermediate filaments in human astrocytomas. Acta Neuropathol (Berl) 70:333–339CrossRefGoogle Scholar
  20. Kennedy PGE, Watkins BA, Thomas DGT, Noble MD (1987) Antigenic expression by cells derived from human gliomas does not correlate with morphological classification. Neuropathol Appl Neurobiol 13:327–347PubMedGoogle Scholar
  21. Kepes JJ (1982) Meningiomas, biology, pathology and differential diagnosis. In: Steinberg SS (ed) Masson monographs in diagnostic pathology. Masson, New York, p 3Google Scholar
  22. Kepes JJ (1986) The histopathology of meningiomas. A reflection of origins and expected behaviour. J Neuropathol Exp Neurol 45:95–107PubMedCrossRefGoogle Scholar
  23. Kepes JJ, Fulling KH, Garcia JH (1982) The clinical significance of “adenoid” formations of neoplastic astrocytes, imitating metastatic carcinomas, in gliosarcomas. A review of five cases. Clin Neuropathol 4:139–150Google Scholar
  24. Kimura T, Budka H, Soler-Federspiel S (1986) An immunocytochemical comparison of the glia-associated proteins, glial fibrillary acidic protein (GFAP) and S-100 protein (S-100p) in human brain tumours. Clin Neuropathol 5:21–27PubMedGoogle Scholar
  25. Mannoji H, Becker LE (1988) Ependymal and choroid plexus tumours. Cytokeratin and GFAP expression. Cancer 61:1377–1385PubMedCrossRefGoogle Scholar
  26. Miettinen M, Lehto VP, Badley RA, Virtanen I (1982) Expression of intermediate filaments in soft tissue sarcomas. Int J Cancer 30:541–546PubMedCrossRefGoogle Scholar
  27. Miettinen M, Clark R, Virtanen I (1986) Intermediate filament in choroid plexus and ependyma and their tumours. Am J Pathol 123:231–240PubMedGoogle Scholar
  28. Mork SJ, Rubinstein LJ, Kepes JJ (1988a) Patterns of epithelial metaplasia in malignant gliomas. I. Papillary formations mimicking medulloepithelioma. J Neuropathol Exp Neurol 47:93–100PubMedCrossRefGoogle Scholar
  29. Mork SJ, Rubinstein LJ, Kepes JJ, Parentes E, Uphoff DF (1988b) Patterns of epithelial metaplasia in malignant gliomas. II. Squamous differentiation of epithelial-like formations in gliosarcomas and glioblastomas. J Neuropathol Exp Neurol 47:101–118PubMedGoogle Scholar
  30. Ng H-K, Lo ST (1989) Cytokeratin immunoreactivity in gliomas. Histopathology 14:359–368PubMedCrossRefGoogle Scholar
  31. Ng H-K, Tse CCH, Lo STH (1988) Ependymomas- and immunohistochemical study of 14 cases. Histopathology 13:462–465PubMedCrossRefGoogle Scholar
  32. Ramon y Cajal S (1972) Histologie del System Nerveux de l’homme et des vertebres, vol 1; 2da reinpression. Consejo Superior de Investigaciones Cientificas, Madrid, pp 256Google Scholar
  33. Roessmann U, Velasco ME, Gambetti P, Autilio-Gambetti L (1983) Vimentin intermediate filaments are increased in human neoplastic astrocytes. J Neuropathol Exp Neurol 42:309CrossRefGoogle Scholar
  34. Rubinstein LJ, Brucher JM (1981) Focal ependymal differentiation in choroid plexus papilloma. An immunoperoxidase study. Acta Neuropathol (Berl) 53:29–33CrossRefGoogle Scholar
  35. Salisbury JRD, Isaacson PG (1985) Synovial sarcoma: an immunohistochemical study. J Pathol 147:49–57PubMedCrossRefGoogle Scholar
  36. Schiffer D, Giordana MT, Mauro A, Magheli A, Germano I, Giaccone G (1986) Immunohistochemical demonstration of vimentin in human cerebral tumours. Acta Neuropathol (Berl) 70:209–219CrossRefGoogle Scholar
  37. Schnitzer J, Franke WW, Schachner M (1981) Immunohistochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult nervous system. J Cell Biol 90:435–447PubMedCrossRefGoogle Scholar
  38. Schwechheimer K, Kartenbeck J, Moll R, Franke WW (1984) Vimentin filament-desmosome cytoskeleton of diverse types of human meningiomas. Lab Invest 51:584–591PubMedGoogle Scholar
  39. Sternberger LA (1979) The unlabelled antibody peroxidase antiperoxidase (PAP) method. In: Immunohistochemistry. Wiley, Chichester, pp 297–321Google Scholar
  40. Taratuto AL, Molina H, Monges J (1983) Choroid plexus tumours in infancy and childhood. Focal ependymal differentiation. An immunoperoxidase study. Acta Neuropathol (Berl) 59:304–308CrossRefGoogle Scholar
  41. Theaker JM, Gatter KC, Esiri MM, Fleming KA (1986) Epithelial membrane antigen and cytokeratin expression by meningiomas: an immunohistological study. J Clin Pathol 39:435–439PubMedCrossRefGoogle Scholar
  42. Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston RE, Pulford K, Cordell J, Cohen B, Wolf-Peters C, Mason DY (1983) Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 309:1275–1281PubMedGoogle Scholar
  43. Whittaker D, Schilkin KB (1984) Diagnosis of pleural malignant mesothelioma in life: a practical approach. J Pathol 143:147–175CrossRefGoogle Scholar
  44. Zülch KJ (1981) Historical development of the classification of brain tumors and new proposal of the World Health Organization (WHO). Neurosurg Rev 4:123–127PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • F. F. Cruz-Sanchez
    • 1
  • M. Garcia-Bachs
    • 2
  • M. L. Rossi
    • 3
  • S. Rodriguez-Prados
    • 1
  • I. Ferrer
    • 2
  • H. B. Coakham
    • 4
  • J. C. Ferreres
    • 5
  • J. Figols
    • 5
  • A. Palacin
    • 1
  1. 1.Neurological Tissue BankUniversity of Barcelona and Department of Pathology, Hospital Clinico Y ProvincialBarcelonaSpain
  2. 2.Department of Pathology, Hospital Principes de EspanaHospitalet de LlobregatBarcelonaSpain
  3. 3.Department of NeuropathologyMidland Centre for Neurosurgery and Neurology (MCNN)BirminghamEngland
  4. 4.Brain Tumour Research Lab, Department of NeurosurgeryFrenchay HospitalBristolEngland
  5. 5.Department of PathologyHospital Marques de ValdecillaSantanderSpain

Personalised recommendations