Virchows Archiv B

, Volume 59, Issue 1, pp 313–319 | Cite as

Methodological approaches to immunohistochemical demonstration ofβ hexosaminidase in human placental and renal tissue with monoclonal antibodies

  • Mohamed Elhassan Elsafi
  • A. Isaksson
  • B. Hultberg
  • I. Hägerstrand
  • U. Stenram


The lysosomal enzyme,β- hexosaminidase (Hex), was studied in full-term human placentas and in renal tissue using monoclonal antibodies raised against Hex purified from human placentas. The immunohistochemical reaction for Hex was pronounced in trophoblastic cells and macrophages of the basal plate and the smooth chorion, but was faint or negative in the amnion as well as in the syncytiotrophoblast and Hofbauer cells of the chorionic villi. The maternal decidual cells of the basal plate were negative. Biochemical enzyme analysis showed the highest activity in basal plate cells (containing trophoblast, decidual cells, macrophages and neutrophils) and a low activity in the chorionic villi. Placental tissue was less positive with monoclonal antibodies specific for Hex A, compared with antibodies reacting with both Hex A and Hex B. Epithelial cells of the renal proximal tubules were positive to the same degree with antibodies recognizing both Hex A and Hex B as well as those recognizing only Hex A.

Key words

β-hexosaminidase Isoenzymes Immunohistochemistry Kidney Lysosomal enzymes Placenta 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Mohamed Elhassan Elsafi
    • 2
  • A. Isaksson
    • 1
  • B. Hultberg
    • 1
  • I. Hägerstrand
    • 2
  • U. Stenram
    • 2
  1. 1.Department of Clinical ChemistryUniversity HospitalLundSweden
  2. 2.Department of PathologyUniversity HospitalLundSweden

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