Growth of elementary blood vessels in diffusion chambers
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The formation of a network of new capillaries from expiants of muscle, omentum, subcutaneous tissue, tendon and cardiac valve tissue in Algire’s diffusion chambers has been studied in different conditions in order to elucidate some of the mechanisms involved in this type of regeneration.
Evidence has been produced to show that new capillaries may arise not only by the sprouting or bulging mechanism from pre-existing capillaries and small vessels of the expiant, but also by autochtonous proliferation of pre-existing endothelia or by differentiation of a fibroblast-like proliferation, independently of any pre-existing vessels. Bundles of proliferated cells form a pre-capillary network which often assumes a syncytial character and lumina are subsequently produced by degeneration of the inner cores of the network, or by shedding of the inner cells of the bundles.
The presence of extravasated blood red cells with the tissue expiants enhances capillary formation. The extravasated blood becomes eventually occluded in the lumen of the new capillary network, and this indicates that the capillary network grows as an open system.
KeywordsVascular Network Cardiac Valve Capillary Network Diffusion Chamber Capillary Formation
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- Benewolenskaja, S. W.: Hematopoiesis in cultures of the embryonic liver of man. Arch. exp. Zellforsch.9, 128–141 (1929).Google Scholar
- Cameron, G., Chambers, R.: Neoplasm studies. III, Organization of cells of human tumors in tissue culture. Amer. J. Cancer30, 115–129 (1937).Google Scholar
- Herzog, G., Schopper, W.: Über das Verhalten der Blutgefäße in der Kultur. Arch. exp. Zellforsch.11, 202–218 (1931).Google Scholar
- Lewis, W. H.: The outgrowth of endothelium and capillaries in tissue culture. Johns Hopk. Hosp. Bull.48, 242–253 (1931).Google Scholar
- Murray, M. R., Stout, A. P.: Cultural characteristics of hemangioendothelioma. Amer. J. Path.20, 277–283 (1944).Google Scholar
- Rienhoff, W. F., Jr.: Development and growth of the metanephros on permanent kidney in chick embryos (8 to 10 days incubation). Johns Hopk. Hosp. Bull.33, 392–406 (1922).Google Scholar
- Schopper, W.: Netzexplantation. Verh. dtsch. path. Ges.24, 25–28 (1929).Google Scholar
- Scriba, K.: Explantationstudien über das Gefäßwachstum bei 9 Tage alten Hühnerembryonen. Arch. exp. Zellforsch.17, 68–77 (1935).Google Scholar
- Törö, E.: Untersuchungen über die Potenz der Endothelzellen bei der Gefäßbildung in der Gewebekultur. Arch. exp. Zellforsch.20, 156–171 (1937).Google Scholar