Virchows Archiv B

, 51:115 | Cite as

The fate of epidermal colcemid-arrested mitoses

  • Olav Hilmar Iversen
  • Peter Krajci
  • Geir Thoresen


This study reports the fate of hairless mouse epidermal basal cells arrested in mitosis by a traditional stathmokinetic dose of 0.15 mg Colcemid. Epidermal basal cells in the S phase were labeled with 30 μCi (3H)TdR i.p. After 1 h, four animals from a cage of eight mice were given 0.15 mg ColcemidR (Fluka) in 0.5 ml saline, and the other four mice were given saline only. Groups of eight mice (four experimental, four controls) were sacrificed 4, 9, 13, 21 and 25 h after (3H)TdR injection (i.e. 3, 8, 12, 16, 20 and 24 h after Colcemid). The following cell kinetic parameters were determined: the number of labeled basal and suprabasal cells, the mean grain count of the labeled cells, the specific activity, the mitotic count, the number of labeled mitoses, the fraction of labeled mitoses curve and the fraction of cells in S and in G2 as determined by flow cytometry. “Labeled paired twins”, i.e. adjoining labeled cells with approximately the same grain count, were also scored.

All the results taken together support the conclusion that cells labeled with (3H)TdR and arrested 1 h later with 0.15 mg Colcemid go through at least one subsequent cell division and thereafter some of them move out into the suprabasal layer at a normal rate. Hence, after this dose of Colcemid, cells arrested in mitosis for some hours do not die, and the Colcemid treatment does not seem to produce hyperploid cells. The study confirms the usefulness of this dose of Colcemid as a convenient tool for cell kinetic studies.

Key words

Colcemid Epidermis Flowcytometry Labeling index Mitosis Tritiated thymidine 


  1. Burton K (1968) Determination of DNA concentration with diphenylamine. Meth Enzymol 12:163–166Google Scholar
  2. Clausen OPF, Lindmo T, Sandnes K, Thorud E (1976) Separation of mouse epidermal basal and differentiating cells for microflow fluorometric measurements. Virchows Arch [Cell Pathol] 20:261–275Google Scholar
  3. Clausen OPF, Thorud E, Bjerknes R, Elgjo K (1979) Circadian rhythms in mouse epidermal basal cell proliferation. Cell Tissue Kinet 12:319–337PubMedGoogle Scholar
  4. Clausen OPF, Thorud E, Elgjo K (1982) Epidermal proliferation characteristics are similar in the pilary canal of mouse hair follicles and in interfollicular epidermis. Virchows Arch [Cell Pathol] 39:259–266CrossRefGoogle Scholar
  5. Dustin P (1934) Cited in Eigsti and Dustin (1955)Google Scholar
  6. Eigsti OJ, Dustin P (1955) Colchicine — in agriculture, medicine, biology and chemistry. Iowa: The Iowa Stage College Press, AmesGoogle Scholar
  7. Göhde W, Dittrich W (1971) Die cytostatische Wirkung von Daunomycin im Impulscytophotometrie-Test. Arzneim Forsch 21:1656–1658Google Scholar
  8. Iversen U, Iversen OH (1967) Cycles of hair growth in hairless mice. Acta Pathol Microbiol Scand 69:60–62Google Scholar
  9. Laerum OD (1969) Oxygen consumption of basal and differentiating cells from hairless mouse epidermis. J Invest Dermatol 52:204–211PubMedGoogle Scholar
  10. Marrs JM, Voorhees JJ (1971) A method for bioassay of an epidermal chalone-like inhibitor. J Invest Dermatol 56:174–181PubMedCrossRefGoogle Scholar
  11. Nome O (1975) Demecolcin and vinblastine sulphate as stathmokinetic agents for different tissues of the hairless mouse. Pathol Eur 10:221–232PubMedGoogle Scholar
  12. Skjaeggestad Y (1964) Experimental epidermal hyperplasia in mice. Relation to carcinogenesis. Acta Pathol Microbiol Scand [Suppl] 169:1–126Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Olav Hilmar Iversen
    • 1
    • 2
  • Peter Krajci
    • 1
    • 2
  • Geir Thoresen
    • 1
    • 2
  1. 1.Institute of PathologyUniversity of Oslo, RikshospitaletOslo 1Norway
  2. 2.Department of Laboratory AnimalsThe National Hospital, RikshospitaletOslo 1Norway

Personalised recommendations