Summary
Leucocytes from syngeneic rats were labeled with tritiated thymidine and donor and recipient rats were connected by a bilateral arteriovenous shunt. Based on the time-dependent label index and labeling intensity, it was concluded that Kupffer cells, the resident macrophages of the liver, have a half-life of 12.4 days and originate from monocytes undergoing one mitosis within 8.4 days after immigration into the liver. The labeled cells were easily identified as Kupffer cells by their selective immunoreactivity with the monoclonal antibody Ki-M2R which is specific for phagocytosing macrophages in the rat. The applicability of combined autoradiography/immunohistochemistry for the identification of other poorly defined macrophage subpopulations is shown.
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References
Alblas B, Van Furth R (1970) Origin, kinetics and characteristics of pulmonary macrophages in the normal steady state. J Exp Med 149:1504–1518
Beelen RHJ, Van’t Veer M, Fluitsma DM, Hoefsmit ECM (1978) Identification of different peroxidatic activity pattern in human macrophages in vivo and in vitro. J Reticuloendothel Soc 24:355–362
Breard J, Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124:1943–1948
Crofton RW, Diesselhoff-den Dulk MMC, Van Furth R (1978) The origin, kinetics, and characteristics of the Kupffer cells in the normal steady state. J Exp Med 148:1–47
Daems WT, Poelmann RE, Brederoo P (1973) Peroxidatic activity in resident peritoneal macrophages and exudate monocytes of the guinea pig after ingestion of latex particles. J Histochem Cytochem 21:93–95
Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ (1983) Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol 130:145–152
Feller AC, Parwaresch MR, Wacker H-H, Radzun HJ, Lennert K (1983) Combined immunohistochemical staining for surface IgD and T-lymphocyte subsets with monoclonal antibodies in human tonsils. Histochem J 15:557–562
Müller-Ruchholtz W, Dettwyler W, Pfeiffer EF (1962) Kontinuierlicher Blutaustausch zwischen unnarkotisierten Ratten mittels doppelter arterio-venöser Kreuztransfusion. Z Ges Exp Med 135:368–376
Parwaresch MR (1976) The human blood basophil. Morphology, origin, kinetics, function and pathology. Springer, Berlin Heidelberg New York
Parwaresch MR, Wacker H-H (1984) Origin and kinetics of resident tissue macrophages. Parabiosis studies with radiolabelled leucocytes. Cell Tissue Kinet 17:25–39
Parwaresch MR, Desaga JF, Nebelin K (1972) Die Kinetik der basophilen Granulozyten im peripheren Blut bei Kaninchen. Blut 27:33–43
Parwaresch MR, Radzun HJ, Hansmann M-L, Peters K-P (1983 a) Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood. Blood 62:585–590
Parwaresch MR, Radzun HJ, Feller AC, Peters K-P, Hansman M-L (1983 b) Peroxidasepositive mononuclear leukocytes as possible precursors of human dendritic reticulum cells. J Immunol 131:2719–2725
Radzun HJ, Parwaresch MR, Feller AC, Hansmann M-L (1984) Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating cells. Am J Pathol 117:441–450
Stein H, Bonk A, Tolksdorf G, Lennert K, Rodt H, Gerdes J (1980) Immunohistochemical analysis of the organization of normal lymphoid tissue and non-Hodgkin’s lymphomas. J Histochem Cytochem 28:746–760
Volkman A (1976) Disparity in origin of mononuclear phagocytes population. J Reticuloendothel Soc 19:249–268
Wacker H-H, Radzun HJ, Parwaresch MR (1985) Ki-M2R, a new specific monoclonal antibody, discriminates tissue macrophages from reticulum cells and monocytes in vivo and in vitro. J Leukocyte Biol 38:509–520
Whitelaw DM (1966) The intravascular lifespan of monocytes. Blood 28:455–464
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Dedicated to Prof. Dr. Dr. h.c. Karl Lennert, Kiel, on the occasion of his 65th birthday
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Wacker, HH., Radzun, H.J. & Parwaresch, M.R. Kinetics of Kupffer cells as shown by parabiosis and combined autoradiographic/immunohistochemical analysis. Virchows Archiv B Cell Pathol 51, 71–78 (1986). https://doi.org/10.1007/BF02899017
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DOI: https://doi.org/10.1007/BF02899017