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Chinese Science Bulletin

, Volume 45, Issue 18, pp 1703–1707 | Cite as

Aberrant activity of Wnt/Frizzled signaling pathway in renal cancer cell lines

  • Tong Zang
  • Liyan Zhuang
  • Zhiwen Zhang
  • Dianqi Xin
  • Yinglu Guo
Notes

Abstract

The expression ofWnt, Wntreceptor-Frizzled, and several other key components in Wnt pathway in renal cancer cell lines was studied. The result of semi-quantitative RT-PCR has shown that the expression level ofWnt5A andhFz5 mRNA were higher in renal cancer cell line (GRC-1) than in normal renal cell line (HK-2). This result has been confirmed byin situ hybridization. The expression of β-catenin protein was obviously higher in GRC-1 than in HK-2 (P< 0.01), but there were no different expressions of its mRNA between 3 lines. The reasons of the overexpression of β-catenin has been investigated by means of immunocytochemistry, SSCP and so on, no mutation ofβ-catenin gene and APC were found. That means that the overexpression ofWnt5A/hFz5 might be the reason of overexpression of β-catenin. It was concluded that the aberrant activity of Wnt pathway might play an important role in renal cell carcinoma.

Keywords

Wnt/Frizzled β-catenin signaling pathway renal cell carcinoma 

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Copyright information

© Science in China Press 2000

Authors and Affiliations

  • Tong Zang
    • 1
  • Liyan Zhuang
    • 1
  • Zhiwen Zhang
    • 2
  • Dianqi Xin
    • 1
  • Yinglu Guo
    • 1
  1. 1.Institute of Urology, First Teaching HospitalBeijing Medical UniversityBeijingChina
  2. 2.Department of PhysiologyBeijing Medical UniversityBeijingChina

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