Folia Microbiologica

, 42:130 | Cite as

Sterol-side chain cleavage by fusants ofAlkaligenes fœcalis andArthrobacter oxydans

  • S. Barthakur
  • M. K. Roy
  • A. C. Ghosh


Protoplasts of a strain ofA. fœcalis incapable of utilizing β-sitosterol as carbon source for growth were fused with protoplasts ofA. oxydans — a strain capable of complete degradation of β-sitosterol. Five fusants showing morphology and pattern of transformation of C-19 steroids identical toA. Fœcalis were selected. Analysis of the fermentation broth containing β-sitosterol showed that the fusants were capable of utilizing β-sitosterol for growth but their pattern of metabolite formation from β-sitosterol was different from that ofA. oxydans. The study revealed that the protoplast fusion technique could be used for intergeneric transfer of genetic determinants linked to partial cleavage of β-sitosterol side chain toA. fœcalis fromA. oxydans.


Sitosterol Intergeneric Transfer Sodium Malate Fusant Strain Sterol Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Choi S.K., Kim H.S., Park Y.H.: Microbial conversion of cholesterol and androstadiene dione by intermittent addition of substrate.Korean J.Appl.Microb.Bioeng. 16, 187–192 (1988).Google Scholar
  2. Dutta R.K., Roy M.K., Singh H.D.: Metabolic blocks in the degradation of β-sitosterol by a plasmid cured strain ofArthrobacter oxydans.J.Basic Microbiol. 32, 167–176 (1992a).PubMedCrossRefGoogle Scholar
  3. Dutta R.K., Roy M.K., Singh H.D.: Role of plasmid pJLI ofArthrobacter oxydans 317 in the degradation of β-sitosterol.J.Basic Microbiol. 33, 317–324 (1992b).CrossRefGoogle Scholar
  4. Goswami P.C., Singh H.D., Hazarika A.K., Barua J.N.: Isolation and characterization of sterol metabolites formed by anArthrobacter sp.Indian J.Biochem.Biophys. 19, 367–369 (1982).PubMedGoogle Scholar
  5. Goswami P.C., Singh H.D., Bhagat S.D., Barua J.N.: Mode of uptake of insoluble solid substrate by micro-organisms: sterol uptake by anArthrobacter sp.Biotechnol.Bio-Eng. 29, 2929–2943 (1983).CrossRefGoogle Scholar
  6. Hesselink P.G.M., Koops K., Harkes M., Githot B.: Growth phase dependence ofMycobacterium sterol side chain cleavage.Proc. Netherl. Biotechnol. Congr. (Britdieret al., Eds), pp. 417–419 (1989).Google Scholar
  7. Jekkel A., Csajagi E., Ilkoy E., Ambrus G.: Genetic recombination by spheroplast fusion of sterol-transformingMycobacterium strains.J.Gen.Microbiol. 135, 1727–1733 (1989).Google Scholar
  8. Kanatani K., Yoshida K., Tahara T., Sakamoto M., Oshimura M.: Intra-specific protoplast fusion ofLactobacillus plantarab.Agric.Biol.Chem. 54, 225–227 (1984).Google Scholar
  9. Katayama M., Holl C.T., Chen N.C., Hiroto T., Kiribuchi T., Funashi S.: A method for fractional determination of soybean sterol in four classes by florisil column chromatography.Agric.Biol.Chem. 38, 1661–1667 (1974).Google Scholar
  10. Kieslich K.: Microbial side chain degradation of sterol.J.Basic Microbiol. 25, 461–474 (1985).PubMedCrossRefGoogle Scholar
  11. Martin C.K.A.:Sterols in Biotechnology (H.J. Rehm, G. Reed, Eds), pp. 79–95. Verlag Chemie, Weinheim 1984.Google Scholar
  12. Rodriguez H., Garcia B., Ancheta O., Sipiczki M.: Formation, regeneration and fusion of protoplast in aCellulomonas strain.Current Microbiol. 23, 265–269 (1991).CrossRefGoogle Scholar
  13. Sarmah U., Roy M.K., Singh H.D.: Steroid transformation by a strain ofArthrobacter oxydans incapable of steroid ring degradation.J.Basic Microbiol. 29, 85–92 (1989).CrossRefGoogle Scholar
  14. Saunders J.R., Doherty A., Humphrey G.O.: Transformation of bacteria by plasmid DNA.Methods Microbiol. 17, 61–95 (1984).CrossRefGoogle Scholar
  15. Shaw P.C., Hartley B.C.: A host vector system for anArthrobacter sp.J.Basic Microbiol. 131, 903–911 (1988).Google Scholar

Copyright information

© Folia Microbiologica 1997

Authors and Affiliations

  • S. Barthakur
    • 1
  • M. K. Roy
    • 1
  • A. C. Ghosh
    • 1
  1. 1.Biochemistry DivisionRegional Research LaboratoryJorhatIndia

Personalised recommendations