Abstract
Cell suspension ofRhizobium leguminosarum bv.viciae D-253,R. leguminosarum bv.viciae D-560 andR. meliloti D-557 were incorporated into sterile diatomaceous earth (DE) and dried at room temperature. Initial numbers of colony-forming units (CFU), expressed as log10, were 8.27, 8.36 and 8.51, respectively. After 5 months of storage the CFU numbers were 0.00, 5.99 and 7.43, respectively.R. meliloti D-557 showed only minor lowering of the CFU number even after 16 months of storage (log10=7.07). After 7 months of storage in DE some single-colony isolates of D-253 produced 10–100 times higher CFU numbers than the original strain. The isolates of D-560 were much more drought-tolerant. The cells of the original strain died after 7 months of storage, log10 of CFU was 6–7 in the isolates. In both strains some of their drought-tolerant isolates had the same specific acetylene-reducing activity of nodule tissue as the original strains. Diatomaceous earth seems to be a prospective carrier for the formulation of bacterization preparations.
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Nečásek, J., Němcová, M., Lisá, L. et al. Drought tolerance ofRhizobium leguminosarum andR. meliloti . Folia Microbiol 38, 320–324 (1993). https://doi.org/10.1007/BF02898601
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DOI: https://doi.org/10.1007/BF02898601