Folia Microbiologica

, Volume 38, Issue 4, pp 320–324 | Cite as

Drought tolerance ofRhizobium leguminosarum andR. meliloti

  • J. Nečásek
  • M. Němcová
  • L. Lisá
  • J. Dusbábková
  • V. Našinec
  • D. Požárková


Cell suspension ofRhizobium leguminosarum bv.viciae D-253,R. leguminosarum bv.viciae D-560 andR. meliloti D-557 were incorporated into sterile diatomaceous earth (DE) and dried at room temperature. Initial numbers of colony-forming units (CFU), expressed as log10, were 8.27, 8.36 and 8.51, respectively. After 5 months of storage the CFU numbers were 0.00, 5.99 and 7.43, respectively.R. meliloti D-557 showed only minor lowering of the CFU number even after 16 months of storage (log10=7.07). After 7 months of storage in DE some single-colony isolates of D-253 produced 10–100 times higher CFU numbers than the original strain. The isolates of D-560 were much more drought-tolerant. The cells of the original strain died after 7 months of storage, log10 of CFU was 6–7 in the isolates. In both strains some of their drought-tolerant isolates had the same specific acetylene-reducing activity of nodule tissue as the original strains. Diatomaceous earth seems to be a prospective carrier for the formulation of bacterization preparations.


Drought Tolerance Diatomaceous Earth Original Strain Rhizobium Population Dinitrogen Fixation 
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Copyright information

© Folia Microbiologica 1993

Authors and Affiliations

  • J. Nečásek
    • 1
  • M. Němcová
    • 2
  • L. Lisá
    • 2
  • J. Dusbábková
    • 1
  • V. Našinec
    • 1
  • D. Požárková
    • 1
  1. 1.Institute of Plant Molecular BiologyAcademy of Sciences of the Czech RepublicČeské Budějovice
  2. 2.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4

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