Biologia Plantarum

, 33:225 | Cite as

Regeneration of plants from leaf mesophyll protoplasts of the tetraploid potato cultivars Xenia and Bintje

  • J. Bříza
  • Iveta Machová


An improved method for the isolation of leaf mesophyll protoplasts ofSolanum tuberosum L. cvs. Xenia and Bintje, their culture and regeneration to plants is described. The improvements involve the culture of nodal stem explants on Schenk and Hildebrandt (SH) medium supplemented with AgNO3 (7.5 mg 11) and Alar 85 (5 mg 1−1), and the pre-treatment of donor plants for 2–3 weeks at a short photoperiod (6 h), a low irradiance (17 (μmol m−2s−1) and a low temperature (20 °C). Regeneration of cell wals was observed within 2–3 d and cell divisions within 7–12 d after resuspending freshly isolated protoplasts in Sidorov et al. (SW) medium. The resulting microcalli were cultured on solid media containing zeatin (1–2,5 mg 1−1) and/or NAA (0.1 and/or 0.01 mg 1−1) and subsequently on medium with BAR (0.25 mg1−1) and GA−3 (0.1 mg 11) for shoot regeneration.


Cellulase Plant Regeneration Zeatin Donor Plant Mesophyll Protoplast 
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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • J. Bříza
    • 1
  • Iveta Machová
    • 1
  1. 1.Institute of Plant Molecular BiologyCzechoslovak Academy of SciencesČeské BudějoviceCzechoslovakia

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