Biologia Plantarum

, Volume 33, Issue 2, pp 135–144 | Cite as

Photoautotrophy established in multiple-shoot cultures of ruta graveolens

  • J. C. Leclerc
  • J. Creche


During the growth of multiple-shoot cultures of Ruta graveolens, oxygen and carbon dioxide exchanges were continuously and simultaneously measured. The shoots subcultured on a medium containing 166 mM glucose showed a marked respiration rate. Even under light, CO2 concentrations reached 4000 to 6000 cm3 m-3. Photosynthesis never compensated for respiration. These cultures were photomixotrophic. A change of respiration and photosynthesis occurred between the 30th and the 32nd day of culture, with a high respiration rate. When the shoots were subcultured on a medium containing 41 mM glucose, it was possible to obtain photoautotrophy after two weeks under high irradiance (150 umol m-2 s-2), and after three weeks under low irradiance (60 μmol m-2 s-1), the CO2 concentrations being 1100 and 600 cm3 m-3 respectively.


Plant Tissue Culture High Irradiance Shoot Culture High Respiration Rate Acridone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Corduan, G.: Autotrophe Gewebekulturen von Rufagraveolens und deren14CO2-Mar- kierungsprodukte. - Planta91: 291–301,1970.CrossRefGoogle Scholar
  2. Corduan, G., Reinhard, E.: Synthesis of volatile oils in tissue cultures of Rutagraveolens. - Phytochemistry11: 917–922,1972.CrossRefGoogle Scholar
  3. Czygan, F. C.: C.: Possibilities for the production of medical substances by plant tissue cultures. - Planta med. (Suppl.): 169–185,1975.Google Scholar
  4. Drawert, F., Berger, R. G., Godelmann, R., Collin, S. Barz, W.: Characterisation of volatile constituents from photomixotrophic cell suspension cultures ofRuta graveolens. - Z. Naturforsch.39c: 525–530, 1984.Google Scholar
  5. Eilert, U., Engel, B., Reinhard, E., Wolters, B.: Acridone epoxides in cell cultures of Ruta species. - Phytochemistry22:14–15,1983.Google Scholar
  6. Fournioux, J. C, Bessis, R.: Influence de la teneur en gaz carbonique sur la morphogenèse de la vigne invitro.- Can. J. Bot.64: 2608–2621,1986.CrossRefGoogle Scholar
  7. Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.- Exp. Cell Res.50:151–158,1968.PubMedCrossRefGoogle Scholar
  8. Hagimori, M., Matsumoto, T., Mikami, Y.: Photoautotrophic culture of undifferentiated cells and shoot forming cultures ofDigitalis purpurea L. - Plant Cell Physiol.25: 1099–1102, 1984.Google Scholar
  9. Heble, M. R.: Multiple shoot cultures: a viable alternativein vitro system for the production of known and new biologically active plant constituents - In: Neuman, K. H., Barz, W., Reinhard, E. (ed.): Primary and Secondary Metabolism of Plant Cell Cultures. Pp. 281–289. Springer Verlag. Berlin -Heidelberg-New York-Tokyo 1985.Google Scholar
  10. Herzbeck, H., Huseman, W.: Photosynthetic carbon metabolism in photoautotrophic cell suspension cultures ofChenopodium rubrum L. - In: Neuman, K. H., Barz, W., Reinhard, E. (ed.): Primary and Secondary Metabolism of Plant Cell Cultures. Pp. 15–23. Springer Verlag. Berlin-Heidelberg-New York-Tokyo 1985.Google Scholar
  11. Ignatev, A. R., Polevaya, V. S.: [Influence of glutamate on photosynthesis inRuta graveolens tissue culture at a limiting CO2 concentration]. In Russ. - Fiziol. Rast.33: 559–565,1986.Google Scholar
  12. Leclerc, J. C, Ab del Rahman, N.: Une crise de croissance du jeune maïs, étudiée avec un nouvel appareillage. - Compt. rend. Acad. Sci. Paris.Sér. III,306: 421–426,1988.Google Scholar
  13. Nato, A., Hoarau, J., Bourdu, R.: The contribution of photosynthetic activity in the growth ofNicotiana tabacum green cell suspension culture. - In: Fujiwara, A. (ed.): Plant Tissue Culture. Pp. 253–254. Mazuren Co., Tokyo 1982.Google Scholar
  14. Neuman, K. M., Bender, L.: Photosynthesis in cell and tissue culture systems. - In: Green, C. E., Somers, D. A., Hackett, W. P., Biesboer, D. D. (ed.): Plant Tissue and Cell Culture. Pp. 151–165. Alan R. Liss, Inc., New York 1987.Google Scholar
  15. Petit-Paly, G., Ramawat, K. G., Chenieux, J. C. Rideau, M.:Ruta greveolens: in vitro production of alkaloids and medicinal compounds. - In: Bajaj, Y. P. S. (ed.): Biotechnology in Agriculture and Forestry. Vol 7. Medicinal and Aromatic Plants II. Pp. 488–505. Springer Verlag, Berlin-Heidelberg- -New York-London-Paris-Tokyo 1989.Google Scholar
  16. Ramawat, K. G., Rideau, M., Chenieux J. C.: Growth and quaternary alkaloid production in differentiating and non differentiating strains ofRuta graveolens. - Phytochemistry24: 441–445, 1905.CrossRefGoogle Scholar
  17. Reinhard, E., Corduan, G., Volk, O. H.: Über Gewebekulturen vonRuta graveolens. - Planta med.16:8–16,1968.PubMedCrossRefGoogle Scholar
  18. Smolov, A. P., Polevaya, V. S., Shumilina, I. N.: [The effect of sucrose and light on the growth and respiration ofrue tissue culture on passing from heterotrophic to photoheterotrophic nutrition]. In, Russ. - Fiziol. Rast.25: 531–535,1978.Google Scholar
  19. Solárová, J., Pospíšilová, J. Catský, J., Šantrůček, J.: Photosynthesis and growth of tobacco plantlets in dependence on carbon supply. - Photosynthetica23: 629–637,1989.Google Scholar
  20. Yamada, Y., Sato, F., Hagimori, M.: Photoautotrophism in green cultured cells. - In: Thorpe, T. A. (ed.): Frontiers of Plant Tissue Culture. Pp. 453–462, IAPTC, Calgary 1978.Google Scholar
  21. Yamada, Y., Sato, F., Watanabe, K.: Photosynthetic carbon metabolism in cultured photoautotrophic cells. - In: Fujiwara, A. (ed): Plant Tissue Culture. Pp. 249–250, Mazuren Co., Tokyo 1982.Google Scholar
  22. Yamaya, T., Ojima, K., Ohira, K.: Studies on the greening of cultured soybean andRuta cells. II: Photosynthetic activities of the cultured green cells. - Soil Sci. Plant Nutr.23: 59–66,1977.Google Scholar

Copyright information

© Academia 1991

Authors and Affiliations

  • J. C. Leclerc
    • 1
  • J. Creche
    • 2
  1. 1.Département Structures et Métabolisme des plantesUniversité Paris-SudOrsayFrance
  2. 2.Département de Biotechnologie végétaleUniversité de ToursToursFrance

Personalised recommendations