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Biologia Plantarum

, Volume 33, Issue 2, pp 135–144 | Cite as

Photoautotrophy established in multiple-shoot cultures of ruta graveolens

  • J. C. Leclerc
  • J. Creche
Article
  • 34 Downloads

Abstract

During the growth of multiple-shoot cultures of Ruta graveolens, oxygen and carbon dioxide exchanges were continuously and simultaneously measured. The shoots subcultured on a medium containing 166 mM glucose showed a marked respiration rate. Even under light, CO2 concentrations reached 4000 to 6000 cm3 m-3. Photosynthesis never compensated for respiration. These cultures were photomixotrophic. A change of respiration and photosynthesis occurred between the 30th and the 32nd day of culture, with a high respiration rate. When the shoots were subcultured on a medium containing 41 mM glucose, it was possible to obtain photoautotrophy after two weeks under high irradiance (150 umol m-2 s-2), and after three weeks under low irradiance (60 μmol m-2 s-1), the CO2 concentrations being 1100 and 600 cm3 m-3 respectively.

Keywords

Plant Tissue Culture High Irradiance Shoot Culture High Respiration Rate Acridone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Academia 1991

Authors and Affiliations

  • J. C. Leclerc
    • 1
  • J. Creche
    • 2
  1. 1.Département Structures et Métabolisme des plantesUniversité Paris-SudOrsayFrance
  2. 2.Département de Biotechnologie végétaleUniversité de ToursToursFrance

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